CloneChecker™ System is a rapid method for screening recombinant bacterial colonies or liquid cultures of colonies for the presence of target plasmid DNA (1). The CloneChecker™ System:
• Screens bacterial colonies for plasmid DNA and insert of interest prior to purification • Screens for inserts >200 bp in plasmids up to 15 kb by size comparison to the original vector or a DNA size marker • Goes from plated colonies through lysis in less than 5 minutes • Allows for supercoiled, restriction digest, or PCR analysis • Works with single-copy plasmids starting with a 2-mm bacterial colony • Includes sufficient reagents for processing 100 bacterial samples by supercoiled analysis (direct size comparison); and 100 samples by restriction endonuclease or PCR analysis
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Sample Type
Bacterial Culture
Final Product Type
Plasmid DNA
For Use With (Application)
Cloning, PCR
High-throughput Compatibility
Not High-throughput Compatible (Manual)
No. of Reactions
100 Preps
Prep Scale
< 100 μg (Small-Scale) Plasmid DNA
Product Line
CloneChecker™
Product Type
Bacterial Colony Screening System
Quantity
100 Preps
Shipping Condition
Room Temperature
Target
Plasmid DNA
Contents & Storage
The CloneChecker™ System includes Yellow, Red, and Green solutions and Loading buffer.