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USP7 Recombinant Human Protein, N-His.GST Tag

Catalog number: 11681H20BL25

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Description

Ubiquitin Carboxyl-Terminal Hydrolase 7 (USP7), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding full length of human USP7 (NP_003461.2) (Met 1-Asn 1102) fused to the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized human P53 at 2 µg/ml (100 µl/well) can bind human USP7 with a linear ranger of 6.4-160 ng/ml.

Formulation: Lyophilized in 50 mM Tris 100 mM NaCl, pH 85% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: Other Proteins
Research Category: Epigenetics, Signal Transduction, Virology
Endotoxin Level: < 1 EU/µg
Expression: Insect
Form: Lyophilized
Gene Aliases: HAUSP, TEF1
Gene Symbol: USP7
Molecular Weight: 156 kDa
Product Line: Sino Biological®
Product Size: 5 x 5 µg
Protein Name: Ubiquitin carboxyl-terminal hydrolase 7
Protein Subtype: Proteases
Protein Tag or Fusion: GST Tag, His Tag
Purity: ≥ 85% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 5 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols