Platinum® Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3´→5´ exonuclease) activity. Platinum® antibody technology provides a simple, automatic, hot-start method that improves PCR specificity (see figure). PCR_x Enhancer Solution is included for higher primer specificity, broader magnesium concentration allowances, broader annealing temperature parameters, and improved thermostability of Platinum® Pfx DNA Polymerase. The PCR_x Enhancer Solution also helps optimize PCR of problematic and/or GC-rich templates. Platinum® Pfx DNA Polymerase provides:

• 26 times higher fidelity than Taq DNA polymerase
• Amplification of fragments up to 12 kb
• Room temperature reaction assemblyApplications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. Platinum® Pfx DNA Polymerase is especially effective when very high levels of fidelity are required.

Unit definition
One unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 min at 74°C.