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Glasgow's MEM (GMEM) This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  11710035

 Related applications:

Mammalian Cell Culture

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Description

Glasgow's MEM (GMEM) was originally developed by Ian McPherson and Michael Stoker as a modification of Eagle's Minimal Essential Medium. It was used to study the genetic factors that affected cell competence. Glasgow's MEM was developed for use with kidney cell lines, such as BHK-21.

This Glasgow's MEM is modified as follows:
WithWithout
• L-glutamine• HEPES
• Phenol Red• Tryptose phosphate broth

The complete formulation is available.

Using Glasgow's MEM
Glasgow's MEM is unique from other media as it contains twice the concentration of amino acids and vitamins compared to the original Basal Medium Eagle, and is used without serum. Glasgow's MEM was originally formulated with 10% tryptose phosphate broth. Glasgow's MEM contains no proteins, lipids, or growth factors. Therefore, Glasgow's MEM requires supplementation with 10% tryptose phosphate broth. Glasgow's MEM uses a sodium bicarbonate buffer system (2.75 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Dual-site cGMP manufacturing
Glasgow's MEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Glasgow's MEM product made in our Scotland facility (21710-025). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
For In Vitro Diagnostic Use. For Research Use Only in China.

Specifications

Form: Liquid
Glucose: High Glucose
Glutamine: L-Glutamine
HEPES Buffer: No HEPES
Phenol Red Indicator: Phenol Red
Product Line: Gibco®
Product Size: 500 mL
Sodium Pyruvate Additive: No Sodium Pyruvate
Volume (Metric): 500 ml
Green Features: Sustainable packaging
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Media formulations

Documents

Manuals & protocols