The RNA UltraSense™ One-Step Quantitative RT-PCR System is specially designed for amplification and real-time quantification of ultra-low abundance transcripts and RNA viruses. The optimized, ultra-concentrated system combines SuperScript® III RT and Platinum® Taq DNA Polymerase to provide greater priming specificity, higher product yields, and detection over a broad dynamic range. The high concentration formulation provides great flexibility with low concentration / high volume RNA samples, and improves performance with targets rich in secondary structure. As little as 25 pg/µl total RNA can be detected with a PCR efficiency of 97% (Figure 1). The RNA UltraSense™ System features:
• SuperScript® III RT for higher temperature cDNA synthesis (up to 60°C) for greater success with RNA secondary structure • Platinum® Taq DNA Polymerase with hot-start technology for improved specificity • Optimized performance with either LUX™ Fluorogenic Primers or dual-labeled fluorogenic probes • Proprietary, ultra-concentrated buffer for addition of large sample volumes (up to 70%) and success with RNA secondary structure • Validated performance on multiple real-time instrument platforms • One-step format for speed, convenience, and less reaction-to-reaction variability
Applications: Real-time quantitative amplification of RNA, including: • RNA viruses • Low-abundance RNA transcripts • Difficult-to-amplify RNA targets • High-value RNA detection and research
For Research Use Only. Not for use in diagnostic procedures.
The RNA UltraSense™ One-Step Quantitative RT-PCR System is supplied with the following components: 250 µl RNA UltraSense™ Enzyme Mix (includes SuperScript® III RT, Platinum® Taq DNA Polymerase, and RNaseOUT™ Ribonuclease Inhibitor); 2 x 1 ml RNA UltraSense™ 5X Reaction Mix; 300 µl 20X Bovine Serum Albumin (BSA), Ultrapure Molecular Biology Grade (5 mg/ml); 1 ml 50-mM magnesium sulfate (MgSO4 ); 100 µl ROX Reference Dye. Sufficient reagents are provided for 100 reactions. Store components at -20°C. Stability can be extended by storing at -80°C. ROX Reference Dye must be stored in the dark.