SuperScript® III Platinum® SYBR® Green One-Step qRT-PCR Kit

      Catalog number: 11736059

      Invitrogen™  Related applications: Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

        Contact us for support ›

      Error loading your content!

        Catalog number
      Select a plan
      Unit size
      		 Price ({{currency}}) Availability Qty
      		{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
      		{{product.availability.message}} —
      Pro add-ons
      Customize this product ›
      Check your on-site stock ›

      Your on-site stock

      ›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
      ›› {{supplyCenter.scName}}(Out of stock)
      ›› {{supplyCenter.scName}}
      This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
      Change selected location › Order non-stocked items
      Back to top

      Description

      The SuperScript® III Platinum® One-Step qRT-PCR Kits combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format. The SuperScript® III Platinum® One-Step qRT-PCR Kit provides sensitive, specific detection with fluorogenic primers and probes, while the SuperScript® III Platinum® SYBR® Green One-Step qRT-PCR Kit offers the ease and convenience of SYBR® Green I detection

      SuperScript® III Platinum® One-Step qRT-PCR Kits provide:

      • SuperScript® III RT for higher temperature cDNA synthesis for greater success with difficult RNA secondary structure
      • Platinum® Taq DNA Polymerase with hot-start technology for improved specificity
      • LUX™ Fluorogenic Primers (Figure 1) or dual-labeled fluorogenic probes (Figure 2) for superior detection performance
      • SYBR® Green I dye for easy and convenient detection (Figure 3)
      • Validated performance on multiple real-time instrument platforms, including rotor-based systems
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      PCR Method: 1-Step qRT-PCR
      Detection Method: SYBR
      Reaction Speed: Standard
      For Use With (Equipment): 7500 System, BioRad MyiQ, BioRad iCycler iQ, BioRad iQ5, Cepheid SmartCycler, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
      Sample Type (General): RNA
      Product Line: Platinum®, SYBR®, SuperScript®
      Product Size: 500 reactions
      Shipping Condition: Dry Ice

      Contents & storage

      • 500 µl SuperScript® III RT/Platinum® Taq Mix
      • 12.5 ml 2X SYBR® Green Reaction Mix
      • 2 × 1 ml Magnesium Sulfate (50 mM)
      • 500 µl ROX Reference Dye (25 µM)
      • 1.3 ml 20X Bovine Serum Albumin (1 mg/ml)

      Store all components at -20°C (-80°C for long-term storage). ROX Reference Dye must be stored in the dark.

      Documents

      Manuals & protocols

      Recommended products