SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit
SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit
Actual product may vary
SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit
SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit
Invitrogen™

SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit

The SuperScript™ III Platinum™ One-Step qRT-PCR Kits combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver preciseRead more
Have Questions?
Change viewbuttonViewtableView
Catalog NumberNo. of Reactions
11736059500 Reactions
11736051100 Reactions
Catalog number 11736059
Price (USD)
2,840.00
Each
Add to cart
No. of Reactions:
500 Reactions
Price (USD)
2,840.00
Each
Add to cart
The SuperScript™ III Platinum™ One-Step qRT-PCR Kits combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format. The SuperScript™ III Platinum™ One-Step qRT-PCR Kit provides sensitive, specific detection with fluorogenic primers and probes, while the SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit offers the ease and convenience of SYBR™ Green I detection

SuperScript™ III Platinum™ One-Step qRT-PCR Kits provide:

• SuperScript™ III RT for higher temperature cDNA synthesis for greater success with difficult RNA secondary structure
• Platinum™ Taq DNA Polymerase with hot-start technology for improved specificity
• LUX™ Fluorogenic Primers (Figure 1) or dual-labeled fluorogenic probes (Figure 2) for superior detection performance
• SYBR™ Green I dye for easy and convenient detection (Figure 3)
• Validated performance on multiple real-time instrument platforms, including rotor-based systems
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
No. of Reactions500 Reactions
PolymeraseTaq DNA Polymerase
Product LinePlatinum™, SYBR™, SuperScript™
Product TypeOne-Step qRT-PCR Kit
Quantity500 reactions
Sample TypeRNA
Shipping ConditionDry Ice
Sufficient For500 Reactions
Detection MethodSYBR
For Use With (Application)Real Time PCR (qPCR)
PCR Method1-step RT-qPCR
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
• 500 μL SuperScript™ III RT/Platinum™ Taq Mix
• 12.5 mL 2X SYBR™ Green Reaction Mix
• 2 × 1 mL Magnesium Sulfate (50 mM)
• 500 μL ROX Reference Dye (25 μM)
• 1.3 mL 20X Bovine Serum Albumin (1 mg/mL)

Store all components at -20°C (-80°C for long-term storage). ROX Reference Dye must be stored in the dark.

Frequently asked questions (FAQs)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.