Our One-Step qRT-PCR Kits with ROX combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient, high-throughput one-step format. One-Step qRT-PCR kits with ROX include:


–SuperScript™ III Reverse Transcriptase: produces higher yields of full-length cDNA for broader gene representation

–Platinum™ Taq DNA Polymerase: activates faster and more completely than other hot-start enzymes to deliver more sensitive amplification (Figure 1)
–ROX reference dye premixed at optimal concentration for ABI instruments



Two kit configurations allow your choice of detection methods:

–SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit provides a highly efficient, cost-effective qRT-PCR system with SYBR Green I detection
–SuperScript™ III Platinum™ One-Step qRT-PCR Kit provides sensitive, specific detection for use with LUX™ fluorogenic primers or dual-labeled probes (e.g. TaqMan™) (Figure 2)


Contents and Storage:
SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit with ROX includes SuperScript™ III RT/Platinum™ Taq Mix, 2X Reaction Mix (containing SYBR™ Green I, ROX Reference Dye, dNTPs, and MgSO₄), and 50 mM MgSO₄. SuperScript™ III Platinum™ One-Step qRT-PCR Kit with ROX includes SuperScript™ III RT/Platinum™ Taq Mix, 2X Reaction Mix (containing ROX Reference Dye, dNTPs and MgSO₄), and 50 mM MgSO₄. Store all components at -20°C. Store ROX Reference dye in the dark. Guaranteed stable for 6 months when properly stored.