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Invitrogen™
SuperScript™ IV CellsDirect™ cDNA Synthesis Kit
The SuperScript IV CellsDirect cDNA Synthesis Kit is optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA. Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in PCR and qPCR.
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| Catalog Number | No. of Reactions |
|---|---|
| 11750150 | 50 Reactions |
| 11750350 | 500 Reactions |
Catalog number 11750150
Price (MXN)
-
No. of Reactions:
50 Reactions
The SuperScript IV CellsDirect cDNA Synthesis Kit is optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA. Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in PCR and qPCR.
Features of the SuperScript IV CellsDirect Synthesis Kit
- Compatible with a wide range of mammalian cell types grown under different treatment conditions
- Single-tube format minimizes reagent loss, sample loss, and handling time
- Total lysate volume can be used in first-strand cDNA synthesis reaction, providing greater yields with a limited number of cells and allowing for detection of rare transcripts
- High-quality cDNA generated for use in a variety of applications, including PCR and qPCR
- Simple protocol takes approximately 37 minutes
- Compatible with TaqMan and SYBR Green qPCR master mixes from various manufacturers
- Optimized performance for 1–10,000 cells per sample, results equivalent to those from purified RNA
Features of the SuperScript IV Reverse Transcriptase included in this kit
- Reduced RNase H activity
- High thermal stability
- High yields of cDNA in the first-strand synthesis reaction for greater sensitivity and enhanced detection of rare transcripts
- Superior tolerance to various RT inhibitors
SuperScript IV reverse transcriptase is a top choice for all RT-PCR and qRT-PCR applications. It is a proprietary MMLV mutant with superior robustness and reliability in RT reactions. It is significantly improved over SuperScript III reverse transcriptase in inhibitor resistance, processivity, and reaction speed, while retaining all the benefits of the previous enzyme, including increased thermostability, highly efficient full-length cDNA synthesis, and reduced RNase activity.
How it works
In traditional RT-PCR, RNA is first isolated from cells in a time-consuming procedure that can lead to a loss of material. Using the SuperScript IV CellsDirect cDNA Synthesis Kit, the cells are lysed and the cDNA is generated from the lysate in a single tube with minimal handling and no sample loss. DNase I treatment is an optional step and can be used to eliminate genomic DNA prior to first-strand synthesis. This kit has been optimized for small cell samples, ranging from 10,000 cells down to a single cell.
Specifications
Final Product TypeFirst-Strand cDNA
FormatKit
No. of Reactions50 Reactions
Optimal Reaction Temperature50°C
Quantity50 reactions
Reaction FormatPremixed Components
Reagent TypeReverse Transcription
Reverse TranscriptaseSuperScript IV
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
Starting MaterialCells
TechniqueReverse Transcription
For Use With (Application)RT-PCR, Real Time PCR (qPCR)
GC-Rich PCR PerformanceHigh
Reaction Speed10 min.
Unit SizeEach
Contents & Storage
• SuperScript IV CellsDirect Lysis Solution (2 x 1.25 mL)
• SuperScript IV CellsDirect Stop Solution (1 x 250 μL)
• Lysis Enhancer (1 x 25 μL)
• Dnase I (1 x 55 μL)
• SuperScript IV RT Master Mix (1 x 400 μL)
• SuperScript IV No RT Control (1 x 400 μL)
Store at –20°C.