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Invitrogen™

SuperScript™ IV CellsDirect™ cDNA Synthesis Kit

Catalog number: 11750350
Invitrogen™

SuperScript™ IV CellsDirect™ cDNA Synthesis Kit

Catalog number: 11750350
Catalog Number
11750350
Unit Size
500 reactions
Price (USD)
Quantity
Catalog Number
Unit Size
50 reactions
Price (USD)
Quantity
Catalog NumberUnit SizePrice (USD)Quantity
11750350500 reactions
1175015050 reactions
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The SuperScript IV CellsDirect cDNA Synthesis Kit is optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA. Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in PCR and qPCR.

Features of the SuperScript IV CellsDirect Synthesis Kit
• Compatible with a wide range of mammalian cell types grown under different treatment conditions
• Single-tube format minimizes reagent loss, sample loss, and handling time
• Total lysate volume can be used in first-strand cDNA synthesis reaction, providing greater yields with a limited number of cells and allowing for detection of rare transcripts
• High-quality cDNA generated for use in a variety of applications, including PCR and qPCR
• Simple protocol takes approximately 37 minutes
• Compatible with TaqMan and SYBR Green qPCR master mixes from various manufacturers
• Optimized performance for 1–10,000 cells per sample, results equivalent to those from purified RNA

Features of the SuperScript IV Reverse Transcriptase included in this kit
• Reduced RNase H activity
• High thermal stability
• High yields of cDNA in the first-strand synthesis reaction for greater sensitivity and enhanced detection of rare transcripts
• Superior tolerance to various RT inhibitors

SuperScript IV reverse transcriptase is a top choice for all RT-PCR and qRT-PCR applications. It is a proprietary MMLV mutant with superior robustness and reliability in RT reactions. It is significantly improved over SuperScript III reverse transcriptase in inhibitor resistance, processivity, and reaction speed, while retaining all the benefits of the previous enzyme, including increased thermostability, highly efficient full-length cDNA synthesis, and reduced RNase activity.

How it works
In traditional RT-PCR, RNA is first isolated from cells in a time-consuming procedure that can lead to a loss of material. Using the SuperScript IV CellsDirect cDNA Synthesis Kit, the cells are lysed and the cDNA is generated from the lysate in a single tube with minimal handling and no sample loss. DNase I treatment is an optional step and can be used to eliminate genomic DNA prior to first-strand synthesis. This kit has been optimized for small cell samples, ranging from 10,000 cells down to a single cell.

Specifications

Format
Kit
GC-Rich PCR Performance
High
Reaction Speed
10 min.
Technique
Reverse Transcription
Green Features
Less Hazardous, Less Waste
Optimal Reaction Temperature
50°C
Reverse Transcriptase
SuperScript IV
Ribonuclease H Activity
Reduced
Shipping Condition
Dry Ice
For Use With (Application)
RT-PCR, Real Time PCR (qPCR)
Final Product Type
First-Strand cDNA
No. of Reactions
500 Reactions
Reaction Format
Premixed Components
Reagent Type
Reverse Transcription
Starting Material
Cells

Contents & Storage

• SuperScript IV CellsDirect Lysis Solution (3 x 9 mL)
• SuperScript IV CellsDirect Stop Solution (2 x 1.25 mL)
• Lysis Enhancer (1 x 250 μL)
• DNase I (1 x 500 μL)
• SuperScript IV RT Master Mix (4 x 1 mL)
• SuperScript IV No RT Control (4 x 1 mL)

Store at –20°C.

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