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Invitrogen™

SuperScript™ III First-Strand Synthesis SuperMix for qRT-PCR

Catalog number: 11752050
Have Questions?
Invitrogen™

SuperScript™ III First-Strand Synthesis SuperMix for qRT-PCR

Catalog number: 11752050
Have Questions?
Catalog Number
11752050
Unit Size
50 rxns
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
250 rxns
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
1175205050 rxns
-
11752250250 rxns
-
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SuperScript III First-Strand Synthesis SuperMix for qRT-PCR provides the high-temperature capability of SuperScript III Reverse Transcriptase in an optimized SuperMix format for the synthesis of first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR). The simple, time-saving reaction set-up uses just two tubes: a 2X reaction mix and an enzyme mix.

Enzyme mix
SuperScript III Reverse Transcriptase, included in the RT enzyme mix, is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme can be used to synthesize cDNA at a temperature range of 42–60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA. RNaseOUT Recombinant Ribonuclease Inhibitor, also included in the enzyme mix, is an RNase inhibitor protein that safeguards against the degradation of target RNA due to ribonuclease contamination of the RNA preparation.

Reaction mix
The 2X RT reaction mix includes oligo(dT)20, random hexamers, MgCl2, and dNTPs in a buffer formulation that has been optimized for qRT-PCR. E. coli RNase H is provided as a separate tube in the kit to remove the RNA template from the cDNA:RNA hybrid molecule after first-strand synthesis. This has been shown to increase sensitivity in qRT-PCR.

Using SuperScript III First-Strand Synthesis SuperMix
This SuperMix formulation can be used to quantify fewer than 10 copies of a target gene in qRT-PCR, with a broad dynamic range that supports accurate quantification of high-copy mRNA from up to 1 μg of total RNA. Reagents are provided for 50 or 250 RT reactions of 20 μL each.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
qPCR
Format
Super Mix
GC-Rich PCR Performance
High
Reaction Speed
30 min.
Technique
Reverse Transcription
Optimal Reaction Temperature
50°C
Reverse Transcriptase
SuperScript III
Shipping Condition
Dry Ice
For Use With (Application)
Real Time PCR (qPCR)
Final Product Type
First-Strand cDNA
No. of Reactions
50 Reactions
Reaction Format
Master Mix
Reagent Type
Reverse Transcription
Starting Material
RNA

Contents & Storage

• 2X RT Reaction Mix (500 μL)
• RT Enzyme Mix (100 μL)
E. coli RNase (50 μL)

Store at –20°C.

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