SuperScript™ IV Single Cell/Low Input cDNA PreAmp Kit

Catalog number: 11752480

SuperScript™ IV Single Cell/Low Input cDNA PreAmp Kit

Catalog number: 11752480
Catalog Number
11752480
Unit Size
480 reactions
Price (USD)
Price: 6,790.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
48 reactions
Price (USD)
Price: 1,212.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
96 reactions
Price (USD)
Price: 2,135.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
192 reactions
Price (USD)
Price: 3,685.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
384 reactions
Price (USD)
Price: 6,400.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
11752480480 reactions
Price: 6,790.00
Your Price:
-
1175204848 reactions
Price: 1,212.00
Your Price:
-
1175209696 reactions
Price: 2,135.00
Your Price:
-
11752192192 reactions
Price: 3,685.00
Your Price:
-
11752384384 reactions
Price: 6,400.00
Your Price:
-
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The Invitrogen SuperScript IV Single Cell/Low Input cDNA PreAmp Kit is designed for efficient cDNA synthesis and amplification directly from intact single cells (1–1,000) or low amounts of total RNA (2 pg–10 ng). It contains all required components to perform cell lysis, reverse transcription (RT), and PCR amplification in a convenient premixed format. A combination of superior enzymes (SuperScript IV Reverse Transcriptase and Platinum SuperFi U DNA Polymerase) enables high yields, sensitivity, and accuracy. Due to the short reaction times and minimal hands-on time, this simple one-tube preamplification protocol can be completed in just over two hours. The high quality global cDNA obtained can be used for next-generation sequencing (NGS) applications or for gene expression analysis by real-time PCR.

Features of the SuperScript IV Single Cell/Low Input cDNA PreAmp Kit include:
Superior sensitivity—easy detection of low abundant targets from as low as single cell or 2 pg of total RNA
High quality cDNA—full-length transcript information with uniform coverage
Streamlined workflow—simple one-tube protocol
Time saving—short lysis and reverse transcription times
Universal—global preamplification compatible with downstream analysis by NGS or real-time PCR

The kit leverages the terminal deoxynucleotidyl transferase (TdT) activity of the SuperScript IV Reverse Transcriptase. Capturing oligonucleotide containing oligo(dT) and adapter sequences is used as primer for selective first strand cDNA synthesis from poly(A)-containing RNAs. When the reverse transcriptase reaches the 5‘ end of the RNA template, TdT activity adds 1–3 extra nucleotides to the cDNA end, enabling template switching and ligation-free incorporation of adapter sequence in the 3‘ end of the resulting cDNA. The high sensitivity and template switching efficiency of SuperScript IV Reverse Transcriptase allow the capture of even low-abundance targets.

Adapter sequences incorporated in both ends of the cDNA serve as primer-binding sites in the subsequent PCR amplification step, allowing global preamplification of the full-length templates. The included Platinum SuperFi U Preamplification Master Mix contains a a superior high-fidelity DNA polymerase that ensures efficient amplification of long templates (up to 10 kb) with no PCR-induced bias or errors.

Specifications

Format
Kit
No. of Reactions
480 Reactions
Polymerase
Platinum SuperFi U DNA Polymerase
Starting Material
Cells (1 to 1,000), or Total RNA (2 pg to 10 ng)
Includes
Reagents for cell lysis, cDNA synthesis and preamplification
Reverse Transcriptase
SuperScript IV Reverse Transcriptase
Storage Requirements
Store at -15° to -25°C
Validated Application
RT-PCR
For Use With (Application)
NGS, qPCR

Contents & Storage

Ten packs, each containing:• 10X Lysis Buffer, 48 μL
• RNase Inhibitor, 19 μL
• Capturing Oligonucleotide, 48 μL
• 4X SuperScript IV cDNA Synthesis Master Mix, 240 μL
• Template Switching Oligonucleotide, 48 μL
• 2X Platinum SuperFi U Preamplification Master Mix, 2 x 1.2 mL
• Preamplification primer, 48 μL
• Water, nuclease-free, 2 x 1.25 mL

Store at -15 to -25°C.