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SuperScript® VILO™ cDNA Synthesis Kit

Catalog number: 11754050

Invitrogen™  Related applications: Reverse Transcription

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Description

The SuperScript® VILO™ cDNA Synthesis Kit provides the high-temperature capability of SuperScript® III Reverse Transcriptase (RT) in an optimized format for generating first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR). VILO is an acronym for variable input, linear output; and the SuperScript®: VILO™ cDNA Synthesis Kit provides the most consistent results for your RT experiments, with unprecedented linearity across the broadest range of input material.

Using the SuperScript® VILO™ cDNA Synthesis Kit
This formulation provides enhanced cDNA synthesis efficiency and can be used with very low and very high amounts of input RNA (up to 2.5 µg total RNA in a 20 µL reaction), giving a linear response in message abundance as measured by qPCR. The 10X SuperScript® Enzyme Mix includes SuperScript® III RT, RNaseOUT™ Recombinant Ribonuclease Inhibitor, and a proprietary helper protein.

SuperScript® III RT
SuperScript® III RT is an engineered version of M-MLV RT with reduced RNase H activity and increased thermal stability. The enzyme can be used to synthesize cDNA at a temperature range of 42–60°C. Because SuperScript® III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA. RNaseOUT™ Recombinant Ribonuclease Inhibitor safeguards against the degradation of target RNA due to ribonuclease contamination.

Optimized buffer
The 5X VILO™ Reaction Mix includes random primers, MgCl2, and dNTPs in a buffer formulation that has been optimized for qRT-PCR.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Reverse Transcriptase: SuperScript® III
Downstream Application: Real-Time Quantitative PCR (qPCR)
Reaction Format: SuperMix or Master Mix
Sample Type (General): RNA
Primer Type: Random Primers
Product Size: 50 x 20 µL reactions
Shipping Condition: Dry Ice
Optimal Reaction Temperature: 42° C

Contents & storage

The SuperScript® VILO™ cDNA Synthesis Kit is supplied with 5X VILO™ Reaction Mix (200 µL) and a 10X SuperScript® III Enzyme Blend (100 µL), which contains: SuperScript® III RT, RNaseOUT™ Recombinant Ribonuclease Inhibitor, and a proprietary helper protein.

Store at -20°C upon receipt.

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Manuals & protocols

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