CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments

Catalog number:  11754100

 Related applications:

Real Time PCR (qPCR)

| Real Time PCR-Based Gene Expression Profiling

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The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

Features of the CellsDirect™ One-Step qRT-PCR Kit include:

Efficiency—Offers significant time and cost savings for increased throughput
Convenience—Enzyme mix includes both SuperScript® III Reverse Transcriptase and Platinum® Taq DNA Polymerase
Sensitivity—No separate RNA isolation step increases sensitivity by minimizing sample loss
Broad Detection Range—Detect targets from samples ranging from a single cell to 10,000 cells
Versatility—Compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)

Streamline Your Workflow
In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect™ One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.

Ideal for Your qRT-PCR Application
CellsDirect™ technology is validated for gene expression studies involving limited samples, and is suitable for validation of RNAi knockdowns. CellsDirect™ One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps (Figure 1). The CellsDirect™ One-Step qRT-PCR Kit may also be used to detect genomic DNA targets.

CellsDirect™ One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g. TaqMan®) or LUX™ fluorogenic primers.

Note: This kit includes 2X Reaction Mix containing ROX™ Reference Dye. An equivalent kit (11753-100), containing a separate tube of ROX™ Reference Dye, is also available.
WARNING: Reproductive Harm -
For Research Use Only. Not for use in diagnostic procedures.


PCR Method: 1-Step qRT-PCR
Detection Method: Primer-Probe Detection
Reaction Speed: Fast, Standard
For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf® MasterCycler® RealPlex
Sample Type (General): Cells
Product Line: CellsDirect™, Platinum®, SuperScript™
Product Size: 100 reactions

Contents & storage

• 10 ml Resuspension Buffer
• 1 ml Lysis Enhancer
• 500 µl DNase I, Amplification Grade (1 U/µl)
• 160 µl 10X DNase I Buffer
• 400 µl 25 mM EDTA
• 100 µl SuperScript® III RT/Platinum® Taq Mix
• 2 × 1.25 ml 2X Reaction Mix with ROX™
• 1 ml 50 mM MgSO4
• 2 ml DEPC-treated water
• 10 µl HeLa Total RNA (10 ng/µl)
The kit is shipped on dry ice and should be stored at -20°C


Manuals & protocols