Informational:Informational:Upgrade to Invitrogen SuperScript™ IV VILO™ Master Mix or SuperScript™ IV VILO™ Master Mix with ezDNase Enzyme—super-fast, super-efficient, and super-high yields.
Invitrogen™

SuperScript™ VILO™ Master Mix

Catalog number: 11755050
Invitrogen™

SuperScript™ VILO™ Master Mix

Catalog number: 11755050
Catalog Number
11755050
Unit Size
50 reactions
Price (USD)
636.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
500 reactions
Price (USD)
4,920.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
250 reactions
Price (USD)
2,840.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
1175505050 reactions
636.00
Your Price:
***
11755500500 reactions
4,920.00
Your Price:
***
11755250250 reactions
2,840.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
Recommendations
SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications. The master mix combines SuperScript III Reverse Transcriptase (RT) and other RT reaction components in a single-tube format to enable reliable first-strand synthesis and high cDNA yields from a wide range of input RNA concentrations. The high RT efficiency of SuperScript III RT and the optimized formulation allow the master mix to deliver high linearity in cDNA synthesis across a broad range of input RNA template for two-step quantitative RT-PCR (qRT-PCR) assays.

Note: For superior cDNA synthesis performance in RT-qPCR applications, we recommend SuperScript IV VILO Master Mix or SuperScript IV VILO Master Mix with ezDNase Enzyme. SuperScript IV VILO Master Mix elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV RT and further optimized buffer. The master mix enables efficient cDNA synthesis at higher temperatures in less time and provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates.

Specifications

Detection Method
Primer-Probe
Form
Liquid
Format
Liquid
GC-Rich PCR Performance
High
No. of Reactions
50 reactions
PCR Method
RT-PCR, RT-qPCR
Size (Final Product)
10 kb or less
Volume
20 μL
Enzyme Function
Reverse Transcriptase
For Use With (Application)
Two-step RT-qPCR
Includes
SuperScript VILO Master Mix containing SuperScript III RT, RNaseOUT Recombinant Ribonuclease Inhibitor, proprietary helper protein, random primers, dNTPs, MgCl2
No. of Tests
50 Reactions
Optimal Reaction Temperature
42° C
Product Line
SuperScript™, VILO™
Product Type
Master Mix
Reverse Transcriptase
SuperScript™ III
Ribonuclease H Activity
Reduced
Shipping Condition
Dry Ice
Storage Requirements
Store in freezer (-5° to -30°C).
Sufficient For
50 Reactions
Template
ssRNA

Contents & Storage

• SuperScript VILO Master Mix containing SuperScript III RT, RNaseOUT Recombinant Ribonuclease Inhibitor, proprietary helper protein, random primers, dNTPs, MgCl2

Store in freezer (-5 to -30°C).

Figures