Product announcement: A new version of cDNA synthesis master mix, SuperScript™ IV VILO™ Master Mix, gives high cDNA yields in the shortest time. Learn more ›

SuperScript® VILO™ Master Mix

Catalog number: 11755250

Invitrogen™  Related applications: Reverse Transcription

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Description

SuperScript® VILO™ Master Mix is a robust reverse transcriptase premix containing everything you need for reverse transcription (RT) in one tube. SuperScript® VILO™ (Variable Input, Linear Output) Master Mix pairs our tried and tested SuperScript® III Reverse Transcriptase with enhanced buffer and RNaseOUT™ Recombinant Ribonuclease Inhibitor to enable reliable first-strand synthesis and higher cDNA yields from a wide range of input RNA concentrations. This versatility helps deliver unprecedented linearity across the broadest range of input material and increased dynamic range for your quantitative RT-PCR (qRT-PCR) assays. Features of the SuperScript® VILO™ Master Mix:

• Sensitive—linearity from 1 pg to 2.5 µg in a 20 µL reaction
Convenient—single-tube format, just add your RNA and go
Flexible—suitable for qPCR detection methods such as SYBR® Green and TaqMan® assays
Powerful—high yields of cDNA perfect for archivingConsistent results from a variety of input RNA concentrations
The SuperScript® VILO™ Master Mix provides the high-temperature capability of SuperScript® III Reverse Transcriptase in an optimized format. The SuperScript® VILO™ Master Mix can generate first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR) with very low and very high amounts of input RNA (up to 2.5 µg total RNA in a 20 µL reaction).

Reliable, RNA-protected, reverse transcripition from total RNA
Because SuperScript® III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA. The SuperScript® VILO™ Master Mix also includes RNaseOUT™ Recombinant Ribonuclease Inhibitor to safeguard against the degradation of target RNA due to ribonuclease contamination. Robust SuperScript® III RT can be used to synthesize cDNA at a temperature range of 42–55°C.

Broad linearity for less template dilutions
Obtain the same relative representation in your cDNA qPCR template, regardless of gene abundance. During normalization, get both your gene of interest and the reference gene in a linear phase for cDNA synthesis even if their abundances differ in the starting material.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Optimal Reaction Temperature: 42° C
Final Product Size(s): 10 kb or less
Reverse Transcriptase: SuperScript® III
Ribonuclease H Activity: Reduced
Form: Liquid
Template: ssRNA
Product Size: 250 reactions
Enzyme Function: Reverse Transcriptase
Shipping Condition: Dry Ice
Number of Reactions: 250 Reactions

Contents & storage

SuperScript® VILO™ Master Mix (200 µL) includes: SuperScript® III RT, RNaseOUT™ , Recombinant Ribonuclease Inhibitor, a proprietary helper protein, random primers, MgCl2, and dNTPs.

Store at -20°C upon receipt.

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Manuals & protocols

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