Gibco™

Ham's F-12 Nutrient Mix

Green features
Catalog number: 11765047
Gibco™

Ham's F-12 Nutrient Mix

Green features
Catalog number: 11765047
Catalog Number
11765047
Unit Size
1,000 mL
Price (USD)
Price: 52.50
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
500 mL
Price (USD)
Price: 33.64
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Availability
***
Quantity
Catalog Number
Unit Size
10 x 500 mL
Price (USD)
Price: 291.00
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Quantity
Catalog Number
Unit Size
6 x 1,000 mL
Price (USD)
Price: 279.00
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Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
117650471,000 mL
Price: 52.50
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***
11765054500 mL
Price: 33.64
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1176506210 x 500 mL
Price: 291.00
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***
117650706 x 1,000 mL
Price: 279.00
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***
Product Overview
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Additional Information
Recommendations
Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Ham's F-12 is modified as follows:
With
• L-glutamine
• Phenol Red

The complete formulation is available.

Using F-12
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
For supply chain continuity, we manufacture F-12 at two separate facilities; one located in Grand Island, NY and the other in Scotland, UK. Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

Specifications

Concentration
1X
Product Type
Ham's F-12 Nutrient Mix
Sterility
Sterile-filtered
Shipping Condition
Room Temperature
Volume (Metric)
1000 ml
Product Line
Gibco™
Form
Liquid
Glutamine
L-Glutamine
Phenol Red Indicator
Phenol Red
Shelf Life
12 Months From Date of Manufacture
Green Features
Sustainable packaging
Quantity
1000 mL
Cell Type
primay rat astrocytes
With Additives
L-Glutamine, Phenol Red
Sodium Pyruvate Additive
Sodium Pyruvate
Cell Line
CHO, COS-7, and rat prostate epithilial cells
Classification
Animal Origin-Free
Solution Type
Ham's F-12 Nutrient Mixture
HEPES Buffer
No HEPES

Contents & Storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture