Ham's F-12 Nutrient Mix
Ham's F-12 Nutrient Mix
인용 및 참조 문헌 (6)
Gibco™

Ham's F-12 Nutrient Mix

Ham's F-12 Nutrient Mixture (F-12)는 CHO (Chinese Hamster Ovary) 세포의 무혈청 단일 세포 plating을 위해 만들어졌습니다. F-12는 CHO 배양의 무혈청자세히 알아보기
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카탈로그 번호수량
1176506210 x 500 mL
11765054500 mL
117650471000 mL
117650706 x 1000 mL
카탈로그 번호 11765062
제품 가격(KRW)
428,000
온라인 행사
Ends: 31-Dec-2025
450,000
할인액 22,000 (5%)
Each
카트에 추가하기
수량:
10 x 500 mL
Customize this product
제품 가격(KRW)
428,000
온라인 행사
Ends: 31-Dec-2025
450,000
할인액 22,000 (5%)
Each
카트에 추가하기
Ham's F-12 Nutrient Mixture (F-12)는 CHO (Chinese Hamster Ovary) 세포의 무혈청 단일 세포 plating을 위해 만들어졌습니다. F-12는 CHO 배양의 무혈청 성장과 chondrocytes 등 기타 포유류 세포의 혈청 보충 성장에 사용되고 있습니다. 라이프 테크놀로지스는 다양한 세포 배양 어플리케이션에 맞는 여러 가지 Gibco™ F-12 modification을 제공합니다. 배지 선택 도구에서 올바른 제형을 찾아보세요.

Ham's F-12 modification:

함유
• L-glutamine
• Phenol Red

완전한 제형을 이용할 수 있습니다.

다른 기본 배지에 비해 F-12는 zinc, putrescine, hypoxanthine, thymidine 등 다양한 성분이 들어있습니다. F-12에서 CHO 세포 무혈청 성장은 개선된 다양한 제형을 제공합니다.

용도
in vitro 진단용. 주의: 인간이나 동물의 치료용으로 사용할 수 없습니다. 이 용도 외의 사용은 지역 법률에 위반될 수 있습니다.


cGMP 제조 품질 시스템
공급망 유지를 위해 라이프테크놀로지스는 뉴욕 Grand Island와 영국 스코틀랜드에 소재한 두 시설에서 Gibco™ F-12를 제조합니다. 두 시설 모두 cGMP 제조 요건에 부합하며 ISO 13485 인증을 가지고 있고 FDA에 의료기기 제조자로 등록되어 있습니다. 뉴욕 시설은 ISO 9001 인증도 가지고 있습니다.

Gibco™ F-12에는 단백질이나 성장인자가 없습니다. 그렇기 때문에 F-12을 사용하려면 일반적으로 10% Fetal Bovine Serum (FBS)를 보충해야 합니다. F-12은 sodium bicarbonate buffer system (1.176 g ⁄ L)을 이용하기 때문에 생리 pH를 유지하기 위해 5-10% CO2가 필요합니다.

본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
사양
세포주CHO, COS-7, and rat prostate epithelial cells
세포 유형Primary Rat Astrocytes
농도1 X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형Ham's F-12 Nutrient Mixture
수량10 x 500 mL
유통 기한12 Months From Date of Manufacture
배송 조건Room Temperature
분류Animal Origin-free
형태Liquid
멸균Sterile-filtered
Sterilization MethodSterile-filtered
첨가제 포함Glutamine, Phenol Red, Sodium Pyruvate
첨가제 없음No HEPES
Unit SizeEach
구성 및 보관
Storage conditions: 2°C to 8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

자주 묻는 질문(FAQ)

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Ham's F-12 Nutrient Mix, 10 x 500 mL FAQs

인용 및 참조 문헌 (6)

인용 및 참조 문헌
Abstract
Structural basis of G protein specificity of human endothelin receptors. A study with endothelinA/B chimeras.
Authors: Takagi Y; Ninomiya H; Sakamoto A; Miwa S; Masaki T;
Journal:J Biol Chem
PubMed ID:7730310
'The endothelin (ET) family of peptides acts via two subtypes of guanine nucleotide-binding regulatory protein (G protein)-coupled receptors termed ETA and ETB. ET-1 stimulated cAMP formation in Chinese hamster ovary (CHO) cells stably expressing human wild-type ETA (CHO/hETA cells) while it inhibited cAMP formation in CHO cells expressing human wild-type ... More
Molecular cloning and functional analysis of the promoter of the human squalene synthase gene.
Authors: Guan G; Jiang G; Koch R L; Shechter I;
Journal:J Biol Chem
PubMed ID:7665618
'We have cloned and characterized the 5''-flanking region of the gene encoding human squalene synthase. We report here the promoter activity of successively 5''-truncated sections of a 1 kilobase of this region by fusing it to the coding region of a luciferase reporter gene. DNA segments of 200 base pairs ... More
Isolation, characterization, and differentiation of human multipotent dermal stem cells.
Authors:Li L, Fukunaga-Kalabis M, Herlyn M
Journal:Methods Mol Biol
PubMed ID:23483399
Skin, as the body's largest organ, has been extensively used to study adult stem cells. Most previous skin-related studies have focused on stem cells isolated from hair follicles and from keratinocytes. Here we present a protocol to isolate multipotent neural crest stem-like dermis-derived stem cells (termed dermal stem cells or ... More
Functional analysis of the human D2 dopamine receptor missense variants.
Authors: Cravchik A; Sibley D R; Gejman P V;
Journal:J Biol Chem
PubMed ID:8824240
The human dopamine D2 receptor gene (DRD2) has three polymorphic variants that predict the amino acid substitutions Val96 --> Ala, Pro310 --> Ser, and Ser311 --> Cys in the receptor protein. We have investigated the ligand binding and signal transduction properties of these human D2 receptor variants by stably expressing ... More
Requirement of tyrosine residues 333 and 338 of the growth hormone (GH) receptor for selected GH-stimulated function.
Authors: Lobie P E; Allevato G; Nielsen J H; Norstedt G; Billestrup N;
Journal:J Biol Chem
PubMed ID:7665593
We have examined the involvement of tyrosine residues 333 and 338 of the growth hormone (GH) receptor in the cellular response to GH. Stable Chinese hamster ovary (CHO) cell clones expressing a receptor with tyrosine residues at position 333 and 338 of the receptor substituted for phenylalanine (CHO-GHR1-638 Y333F, Y338F) ... More
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