Invitrogen™

ezDNase™ Enzyme

Catalog number:  11766051

Related applications:

Reverse Transcription

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Description

Invitrogen™ ezDNase™ Enzyme is a recombinant double-strand-specific DNase for the fast removal of contaminating genomic DNA from RNA preparations. It cleaves phosphodiester bonds in double-stranded DNA to yield 2–8 bp oligonucleotides with 5’-phosphate and 3’-hydroxyl termini.

Features of ezDNase Enzyme include:
• Efficient and fast genomic DNA removal
• Highly specific—no impact on RNA, cDNA, or primers in RT reactions

ezDNase Enzyme's high specificity for double-stranded DNA enables efficient and fast genomic DNA removal without reduction in the quality or quantity of RNA or single-stranded DNA present in the reaction such as cDNA and primers. ezDNase Enzyme is heat-labile and so can be easily deactivated by heat treatment at moderate temperature (55°C). These features make ezDNase a superior choice for genomic DNA removal prior to reverse transcription reactions.

When used in combination with the SuperScript™ IV VILO™ Master Mix or other Invitrogen reverse transcription reagents, ezDNase Enzyme helps significantly reduce the possibility of cDNA synthesis being compromised by genomic DNA and reduce variation in RT-qPCR quantitation caused by RNA loss or damage during conventional DNase treatment.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Description: ezDNase
Enzyme: DNase
Product Line: ezDNase™
Shipping Condition: On dry ice
No. of Reactions: 50 reactions

Contents & storage

• ezDNase Enzyme, 50 µL
• ezDNase Buffer (10X), 100 µL
• Nuclease-free water, 1.25 mL

Store at -5 to -30°C.