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Invitrogen™

SuperScript™ IV VILO™ Master Mix

Catalog number: 11766500
Invitrogen™

SuperScript™ IV VILO™ Master Mix

Catalog number: 11766500
SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications. The inclusion of ezDNase enzyme further accelerates the RT-qPCR workflow through an extremely simplified genomic DNA removal step.
 
Catalog Number
11766500
Unit Size
Each
Includes
Kit with ezDnase
No. of Reactions
500 Reactions
Price (USD)
Price: 5,820.00
Your price:
Availability
-
Full specifications
Quantity
Detection MethodqPCR
FormatMaster Mix
GC-Rich PCR PerformanceHigh
Reaction Speed10 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C
Reverse TranscriptaseSuperScript IV
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Concentration5X
Final Product TypeFirst-Strand cDNA
No. of Reactions500 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 10 kb
Starting MaterialRNA
IncludesKit with ezDnase
Unit SizeEach
Catalog Number
Unit Size
Each
Includes
Kit only
No. of Reactions
50 Reactions
Price (USD)
Price: 738.00
Your price:
Availability
-
Full specifications
Quantity
Detection MethodqPCR
FormatMaster Mix
GC-Rich PCR PerformanceHigh
Reaction Speed10 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C
Reverse TranscriptaseSuperScript IV
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Concentration5X
Final Product TypeFirst-Strand cDNA
No. of Reactions50 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 10 kb
Starting MaterialRNA
IncludesKit only
Unit SizeEach
Catalog Number
Unit Size
Each
Includes
Kit only
No. of Reactions
500 Reactions
Price (USD)
Price: 5,480.00
Your price:
Availability
-
Full specifications
Quantity
Detection MethodqPCR
FormatMaster Mix
GC-Rich PCR PerformanceHigh
Reaction Speed10 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C
Reverse TranscriptaseSuperScript IV
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Concentration5X
Final Product TypeFirst-Strand cDNA
No. of Reactions500 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 10 kb
Starting MaterialRNA
IncludesKit only
Unit SizeEach
Catalog Number
Unit Size
Each
Includes
Kit with ezDnase
No. of Reactions
50 Reactions
Price (USD)
Price: 778.00
Your price:
Availability
-
Full specifications
Quantity
Detection MethodqPCR
FormatMaster Mix
GC-Rich PCR PerformanceHigh
Reaction Speed10 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C
Reverse TranscriptaseSuperScript IV
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Concentration5X
Final Product TypeFirst-Strand cDNA
No. of Reactions50 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 10 kb
Starting MaterialRNA
IncludesKit with ezDnase
Unit SizeEach
Showing 4 of 4
Catalog NumberSpecificationsUnit SizeIncludesNo. of ReactionsPrice (USD)AvailabilityQuantity
11766500Full specifications
Each Kit with ezDnase500 Reactions
Price: 5,820.00
Your price:
-
Detection MethodqPCR
FormatMaster Mix
GC-Rich PCR PerformanceHigh
Reaction Speed10 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C
Reverse TranscriptaseSuperScript IV
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Concentration5X
Final Product TypeFirst-Strand cDNA
No. of Reactions500 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 10 kb
Starting MaterialRNA
IncludesKit with ezDnase
Unit SizeEach
11756050Full specifications
Each Kit only50 Reactions
Price: 738.00
Your price:
-
Detection MethodqPCR
FormatMaster Mix
GC-Rich PCR PerformanceHigh
Reaction Speed10 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C
Reverse TranscriptaseSuperScript IV
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Concentration5X
Final Product TypeFirst-Strand cDNA
No. of Reactions50 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 10 kb
Starting MaterialRNA
IncludesKit only
Unit SizeEach
11756500Full specifications
Each Kit only500 Reactions
Price: 5,480.00
Your price:
-
Detection MethodqPCR
FormatMaster Mix
GC-Rich PCR PerformanceHigh
Reaction Speed10 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C
Reverse TranscriptaseSuperScript IV
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Concentration5X
Final Product TypeFirst-Strand cDNA
No. of Reactions500 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 10 kb
Starting MaterialRNA
IncludesKit only
Unit SizeEach
11766050Full specifications
Each Kit with ezDnase50 Reactions
Price: 778.00
Your price:
-
Detection MethodqPCR
FormatMaster Mix
GC-Rich PCR PerformanceHigh
Reaction Speed10 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C
Reverse TranscriptaseSuperScript IV
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Concentration5X
Final Product TypeFirst-Strand cDNA
No. of Reactions50 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 10 kb
Starting MaterialRNA
IncludesKit with ezDnase
Unit SizeEach
Showing 4 of 4

SuperScript IV VILO Master Mix features include:
• Fast RT reactions in just 10 minutes
• Convenient one-tube cDNA reaction master mix for two-step RT-qPCR
• High yields reduce Ct values by more than two cycles ahead of all other reverse transcription reagents
• Efficient reaction even with low template amounts and suboptimal purity samples
• gDNA removal in as few as two minutes (SSIV VILO Master Mix with ezDNase only)

SSIV VILO Master Mix elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV Reverse Transcriptase and further optimized buffer. These components enable efficient cDNA synthesis at higher temperatures and in less time. SSIV VILO master mix provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates. It is your new tool for more efficient and reproducible RT-qPCR.

SuperScript IV VILO Master Mix with ezDNase is available for better removal of genomic DNA and to further accelerate the RT-qPCR workflow. The extremely simplified genomic DNA removal step dramatically reduces the time of the entire reverse transcription protocol and reduces possible variation in gene expression due to RNA loss or damage during conventional DNase treatment.

SSIV VILO master mix results in more cDNA in less time with less pipetting, less variation, less reaction inhibition, less gDNA interference, and with less sample than other cDNA synthesis kits or master mixes for RT-qPCR. Additional ezDNase may also be purchased separately.

Source
Purified from E. coli expressing the pol gene of M-MLV, modified to increase thermostability and half-life, processivity, inhibitor resistance, and to reduce RNase H activity

Performance and quality testing
Assayed for endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease, as well as yield and length of cDNA product

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

• SuperScript IV VILO Master Mix (2 x 1 mL)
• SuperScript IV VILO Master Mix 'No RT' Control (1 mL)
• ezDNase Enzyme (500 μL)
• 10X ezDNase Buffer (1 mL)
• Nuclease-free water (1.25 mL)

Store at –5 to –30°C.

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