Search
Invitrogen™
SuperScript V VILO Master Mix, 1250 rxns
Invitrogen™ SuperScript™ V VILO™ Master Mix is a ready-to-use master mix designed for fast, sensitive, and reproducible cDNA synthesis in two-step RT-qPCR applications.
Have Questions?
Change view
| Catalog Number | No. of Reactions | Includes |
|---|---|---|
| 11800001 | 50 Reactions | SuperScript V VILO Master Mix, supplied with No RT Control and Nuclease-Free Water |
| 11800002 | 500 Reactions | SuperScript V VILO Master Mix, supplied with No RT Control and Nuclease-Free Water |
| 11800003 | 2000 Reactions | SuperScript V VILO Master Mix, supplied with No RT Control and Nuclease-Free Water |
| 11800111 | 50 Reactions | SuperScript V VILO Master Mix, supplied with No RT Control, ezDNase Master Mix and Nuclease-Free Water |
| 11800112 | 500 Reactions | SuperScript V VILO Master Mix, supplied with No RT Control, ezDNase Master Mix and Nuclease-Free Water |
| 11800113 | 2000 Reactions | SuperScript V VILO Master Mix, supplied with No RT Control, ezDNase Master Mix and Nuclease-Free Water |
| 11800004 | 1250 Reactions | SuperScript V VILO Master Mix |
Catalog number 11800001
Price (MXN)
-
No. of Reactions:
50 Reactions
Includes:
SuperScript V VILO Master Mix, supplied with No RT Control and Nuclease-Free Water
Invitrogen™ SuperScript™ V VILO™ Master Mix is a ready-to-use master mix designed for fast, sensitive, and reproducible cDNA synthesis in two-step RT-qPCR applications. Powered by SuperScript™ V Reverse Transcriptase, the optimized master mix combines robust performance across a broad range of RNA samples with simplified reaction setup, enhanced reagent stability, and greater workflow flexibility. SuperScript V VILO Master Mix is also available with ezDNase Master Mix for fast and convenient genomic DNA removal prior to reverse transcription.
Features of SuperScript V VILO Master Mix include:
- Broad RNA input range—supports sensitive cDNA synthesis from 0.1 pg to 5 μg of total RNA
- Robust performance with challenging RNA—efficient cDNA synthesis from highly structured, GC-rich, degraded and inhibitor-containing RNA
- Fast cDNA synthesis—complete reverse transcription in as little as 1-10 minutes
- Enhanced reagent stability—supports ambient shipping and room-temperature storage for up to 1 month
- Simple, flexible workflow—ready-to-use format simplifies reaction setup, while room-temperature assembly and extended stability of assembled reactions enable greater workflow flexibility
SuperScript V VILO Master Mix combines SuperScript V RT, RNase inhibitor, buffer, dNTPs, and an optimized blend of oligo(dT) and random primers in a single formulation, simplifying reaction setup and helping reduce variability. Its combination of fast cDNA synthesis, robust performance, and flexible room-temperature handling makes it well suited for routine, high-throughput, and automated RT-qPCR workflows.
SuperScript V VILO Master Mix with gDNA Removal allows streamlined genomic DNA removal step. The dsDNA-specific ezDNase enzyme removes contaminating genomic DNA in as little as 2 minutes without affecting RNA, further simplifying the two-step RT-qPCR workflow.
A DTT-free, animal origin-free formulation combined with reduced cold chain requirements further simplifies reagent handling and supports more sustainable workflows.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Final Product TypeFirst-Strand cDNA
FormatMaster Mix
IncludesSuperScript V VILO Master Mix, supplied with No RT Control and Nuclease-Free Water
Inhibitor Tolerance (Simple)High
No. of Reactions50 Reactions
Optimal Reaction Temperature50°
Product TypeReverse Transcription Reagent
Reagent TypeReverse Transcription
Reverse TranscriptaseSuperScript V
Ribonuclease H ActivityReduced
Sensitivity0.1 pg total RNA
Shipping ConditionDry Ice, Wet Ice, Ambient
Size (Final Product)Up to 10 kb
Starting MaterialRNA
TechniqueReverse Transcription
ThermostabilityUp to 65°
Concentration5X
Detection MethodqPCR, NGS, PCR
For Use With (Application)Gene Expression, RT-PCR, Real Time PCR (qPCR), cDNA Synthesis
FormLiquid
GC-Rich PCR PerformanceHigh
Label or DyeNot Applicable
PCR Method2-step RT-qPCR, RT-PCR
Reaction Speed10 min.
Unit SizeEach
Contents & Storage
• SuperScript V VILO Master Mix (200 μL)
• SuperScript V VILO No RT Control (100 μL)
• Nuclease-free water (1.25 mL)
Store all contents at:
• -20°C for long-term storage until expiry date• +5°C for up to 5 months
• Room temperature for up to 1 months
• SuperScript V VILO No RT Control (100 μL)
• Nuclease-free water (1.25 mL)
Store all contents at:
• -20°C for long-term storage until expiry date• +5°C for up to 5 months
• Room temperature for up to 1 months