Invitrogen™

SuperScript V VILO Master Mix

Name: SuperScript V VILO Master Mix
SKU: 11800003

Invitrogen™ SuperScript™ V VILO™ Master Mix is a ready-to-use master mix designed for fast, sensitive, and reproducible cDNA synthesis in two-step RT-qPCR applications.

Cambiar vista

Número de catálogo	N.º de reacciones	Incluye
11800003	2000 Reactions	SuperScript V VILO Master Mix, No RT Control, Water
11800001	50 Reactions	SuperScript V VILO Master Mix, No RT Control, Water
11800002	500 Reactions	SuperScript V VILO Master Mix, No RT Control, Water
11800111	50 Reactions	SuperScript V VILO Master Mix, No RT Control, ezDNase Master Mix, Water
11800112	500 Reactions	SuperScript V VILO Master Mix, No RT Control, ezDNase Master Mix, Water
11800113	2000 Reactions	SuperScript V VILO Master Mix, No RT Control, ezDNase Master Mix, Water
11800004	1250 Reactions	SuperScript V VILO Master Mix only

7 opciones

Número de catálogo 11800003

Precio (MXN)

N.º de reacciones:

2000 Reactions

Incluye:

SuperScript V VILO Master Mix, No RT Control, Water

Invitrogen™ SuperScript™ V VILO™ Master Mix is a ready-to-use master mix designed for fast, sensitive, and reproducible cDNA synthesis in two-step RT-qPCR applications. Powered by SuperScript™ V Reverse Transcriptase, the optimized master mix combines robust performance across a broad range of RNA samples with simplified reaction setup, enhanced reagent stability, and greater workflow flexibility. SuperScript V VILO Master Mix is also available with ezDNase Master Mix for fast and convenient genomic DNA removal prior to reverse transcription.

Features of SuperScript V VILO Master Mix include:

Broad RNA input range—supports sensitive cDNA synthesis from 0.1 pg to 5 μg of total RNA
Robust performance with challenging RNA—efficient cDNA synthesis from highly structured, GC-rich, degraded and inhibitor-containing RNA
Fast cDNA synthesis—complete reverse transcription in as little as 1-10 minutes
Enhanced reagent stability—supports ambient shipping and room-temperature storage for up to 1 month
Simple, flexible workflow—ready-to-use format simplifies reaction setup, while room-temperature assembly and extended stability of assembled reactions enable greater workflow flexibility

SuperScript V VILO Master Mix combines SuperScript V RT, RNase inhibitor, buffer, dNTPs, and an optimized blend of oligo(dT) and random primers in a single formulation, simplifying reaction setup and helping reduce variability. Its combination of fast cDNA synthesis, robust performance, and flexible room-temperature handling makes it well suited for routine, high-throughput, and automated RT-qPCR workflows.

SuperScript V VILO Master Mix with gDNA Removal allows streamlined genomic DNA removal step. The dsDNA-specific ezDNase enzyme removes contaminating genomic DNA in as little as 2 minutes without affecting RNA, further simplifying the two-step RT-qPCR workflow.

A DTT-free, animal origin-free formulation combined with reduced cold chain requirements further simplifies reagent handling and supports more sustainable workflows.

For Research Use Only. Not for use in diagnostic procedures.

Especificaciones

Tipo de producto finalFirst-Strand cDNA

FormatoMaster Mix

IncluyeSuperScript V VILO Master Mix, No RT Control, Water

Inhibitor Tolerance (Simple)High

N.º de reacciones2000 Reactions

Temperatura óptima de reacción50°C

Tipo de productoReverse Transcription Reagent

Tipo de reactivoReverse Transcription

Transcriptasa inversaSuperScript V

Actividad de la ribonucleasa HReduced

Sensibilidad0.1 pg total RNA

Condiciones de envíoDry Ice, Wet Ice, Ambient

Tamaño (producto final)Up to 10 kb

Material de partidaRNA

TécnicaReverse Transcription

TermoestabilidadUp to 65°

Concentración5X

Método de detecciónqPCR, NGS, PCR

Para utilizar con (aplicación)Gene Expression, Real Time PCR (qPCR), RT-PCR, cDNA Synthesis

FormularioLiquid

GC-Rich PCR PerformanceHigh

Etiqueta o tinteNot Applicable

Método de PCR2-step RT-qPCR, RT-PCR

Velocidad de reacción10 min.

Unit SizeEach

Contenido y almacenamiento

• Four 500 reaction packages of SuperScript V VILO Master Mix (Cat. No. 11800002)

Store all contents at:
• -20°C for long-term storage until expiry date
• +5°C for up to 5 months
• Room temperature for up to 1 month

Preguntas frecuentes

What are the main differences between Invitrogen SuperScript V Reverse Transcriptase and Invitrogen SuperScript V VILO Master Mix?

Invitrogen SuperScript V Reverse Transcriptase is supplied as a stand-alone enzyme with reaction buffer and supports flexible reaction optimization. Invitrogen SuperScript V VILO Master Mix combines all required reaction components into a single formulation to simplify workflow setup and reduce pipetting steps.

Can Invitrogen SuperScript V Reverse Transcriptase products support automated workflows?

Yes. Invitrogen SuperScript V Reverse Transcriptase products include features such as room-temperature reaction setup, benchtop stability of assembled reactions, and simplified workflow options that can support automated and high-throughput workflows.

Can Invitrogen SuperScript V Reverse Transcriptase products support low-input RNA workflows?

Yes. Invitrogen SuperScript V Reverse Transcriptase products are designed to support reverse transcription from low-input RNA samples, including inputs as low as 0.1 pg of total RNA.

Which Invitrogen SuperScript V Reverse Transcriptase product should I choose for RT-qPCR workflows?

For two-step RT-qPCR workflows, Invitrogen SuperScript V VILO Master Mix reagent (Cat. No. 11800001) provides an all-in-one formulation that simplifies reaction setup. Users who prefer custom primer selection may choose Invitrogen SuperScript V VILO Master Mix, Primer-Free (Cat. No. 11900001) or Invitrogen SuperScript V Reverse Transcriptase (Cat. No. 19090050).

Can reaction volumes be scaled when using Invitrogen SuperScript V Reverse Transcriptase products?

Yes. Reaction volumes may be scaled up to 100 µL when using Invitrogen SuperScript V Reverse Transcriptase products provided that reagent ratios are maintained and workflow performance is verified.

View all SuperScript V VILO Master Mix FAQs