Gateway™ pDEST™14 Vector

인용 및 참조 문헌 (3)

Invitrogen™

Gateway™ pDEST™14 Vector

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast,자세히 알아보기

카탈로그 번호	수량
11801016	6 μg

카탈로그 번호 11801016

제품 가격(KRW)

598,000

온라인 행사

Ends: 31-Dec-2025

703,000

할인액 105,000 (15%)

카트에 추가하기

제품 가격(KRW)

598,000

온라인 행사

Ends: 31-Dec-2025

703,000

할인액 105,000 (15%)

카트에 추가하기

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available.

Additional materials required, available separately: Gateway™ entry clone, Gateway™ LR Clonase™ enzyme mix, and reaction buffer.

For Research Use Only. Not for use in diagnostic procedures.

항생제 내성 박테리아Ampicillin (AmpR)

구성 또는 유도성 시스템Inducible

제품 유형Expression Vector

수량6 μg

선택 제제(진핵)None

벡터pDEST, Gateway T7 Vectors

클로닝 방법Gateway

제품라인Gateway, pDEST

프로모터T7

단백질 태그Untagged

Unit SizeEach

구성 및 보관

All destination vectors are provided lyophilized and supercoiled.

자주 묻는 질문(FAQ)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Gateway™ pDEST™14 Vector FAQs

인용 및 참조 문헌 (3)

인용 및 참조 문헌

Abstract

Ubiquitination of a novel deubiquitinating enzyme requires direct binding to von Hippel-Lindau tumor suppressor protein.

Authors: Li Zaibo; Na Xi; Wang Dakun; Schoen Susan R; Messing Edward M; Wu Guan;

Journal:J Biol Chem

PubMed ID:11739384

'von Hippel-Lindau (VHL) disease is a hereditary cancer syndrome caused by germline mutations of the VHL gene. Recent studies suggest that VHL protein (pVHL) is a component of an E3 ubiquitin ligase, but the detailed biological function of pVHL remains to be determined. To further elucidate the biological functions of ... More

Molecular chaperone Hsp90 associates with resistance protein N and its signaling proteins SGT1 and Rar1 to modulate an innate immune response in plants.

Authors:Liu Y, Burch-Smith T, Schiff M, Feng S, Dinesh-Kumar SP,

Journal:J Biol Chem

PubMed ID:14583611

SGT1 and Rar1 are important signaling components of resistance (R) gene-mediated plant innate immune responses. Here we report that SGT1 and Rar1 associate with the molecular chaperone Hsp90. In addition, we show that Hsp90 associates with the resistance protein N that confers resistance to tobacco mosaic virus. This suggests that ... More

Serinc, an activity-regulated protein family, incorporates serine into membrane lipid synthesis.

Authors:Inuzuka M, Hayakawa M, Ingi T,

Journal:J Biol Chem

PubMed ID:16120614

Cell membranes contain various transporter proteins, some of which are responsible for transferring amino acids across membrane. In this study, we report another class of carrier proteins, termed Serinc1-5, that incorporates a polar amino acid serine into membranes and facilitates the synthesis of two serine-derived lipids, phosphatidylserine and sphingolipids. Serinc ... More