Gateway™ pDEST™8 Vector

Citas y referencias (5)

Invitrogen™

Gateway™ pDEST™8 Vector

Para adaptarse a todas sus necesidades de expresión, Invitrogen ofrece vectores de destino Gateway™ de vanguardia de destino para laMás información

Número de catálogo	Cantidad
11804010 también denominado 11804-010	6 μg

Número de catálogo 11804010

también denominado 11804-010

Precio (MXN)

Cantidad:

Para adaptarse a todas sus necesidades de expresión, Invitrogen ofrece vectores de destino Gateway™ de vanguardia de destino para la expresión en célula de E. coli, insecto, levadura o mamífero, así como para la producción de proteína nativa o proteínas de fusión N o C-terminal. Los vectores de destino Gateway™ tienen sitios attR para la recombinación con cualquier fragmento flanqueado por attL, independientemente de si se trata de un clon de entrada o de un clon Ultimate™ RF. La siguiente tabla enumera la amplia gama de vectores de destino disponibles.

Materiales adicionales necesarios, disponibles por separado: Clon de entrada Gateway™, mezcla de enzimas Gateway™ LR Clonase™ y tampón de reacción.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Tipo de productoVector de expresión

Cantidad6 μg

VectorpDEST, vectores de baculovirus Gateway

Método de clonaciónGateway

Línea de productosGateway

PromotorPoliedrina

Etiqueta de proteínaSin etiquetar

Unit SizeEach

Contenido y almacenamiento

Todos los vectores de destino se suministran liofilizados y superenrollados.

Preguntas frecuentes

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Gateway™ pDEST™8 Vector FAQs

Citations & References (5)

Citations & References

Abstract

Target-induced formation of neuraminidase inhibitors from in vitro virtual combinatorial libraries.

Authors: HochgÃ¼rtel Matthias; Kroth Heiko; Piecha Dorothea; Hofmann Michael W; Nicolau Claude; Krause Sonja; Schaaf Otmar; Sonnenmoser Gabriele; Eliseev Alexey V;

Journal:Proc Natl Acad Sci U S A

PubMed ID:11891312

'Neuraminidase, a key enzyme responsible for influenza virus propagation, has been used as a template for selective synthesis of small subsets of its own inhibitors from theoretically highly diverse dynamic combinatorial libraries. We show that the library building blocks, aldehydes and amines, form significant amounts of the library components resulting ... More

Enhanced production of green fluorescent fusion proteins in a baculovirus expression system by addition of secretion signal.

Authors:Katagiri Y, Ingham KC.

Journal:Biotechniques

PubMed ID:12139250

All Six Modules of the Gelatin-binding Domain of Fibronectin Are Required for Full Affinity.

Authors:Katagiri Y, Brew SA, Ingham KC,

Journal:J Biol Chem

PubMed ID:12538576

The gelatin-binding sites of fibronectin are confined to a 42-kDa region having four type I and two type II modules in the following order: I(6)-II(1)-II(2)-I(7)-I(8)-I(9). To determine the relative importance of each module for recognition of gelatin, recombinant green fluorescent fusion proteins were prepared in which individual modules or groups ... More

Cleavage of von Willebrand Factor Requires the Spacer Domain of the Metalloprotease ADAMTS13.

Authors:Zheng X, Nishio K, Majerus EM, Sadler JE,

Journal:J Biol Chem

PubMed ID:12791682

ADAMTS13 consists of a reprolysin-type metalloprotease domain followed by a disintegrin domain, a thrombospondin type 1 motif (TSP1), Cys-rich and spacer domains, seven more TSP1 motifs, and two CUB domains. ADAMTS13 limits platelet accumulation in microvascular thrombi by cleaving the Tyr1605-Met1606 bond in von Willebrand factor, and ADAMTS13 deficiency causes ... More

DNA cloning using in vitro site-specific recombination.

Authors: Hartley J L; Temple G F; Brasch M A;

Journal:Genome Res

PubMed ID:11076863

As a result of numerous genome sequencing projects, large numbers of candidate open reading frames are being identified, many of which have no known function. Analysis of these genes typically involves the transfer of DNA segments into a variety of vector backgrounds for protein expression and functional analysis. We describe ... More