Gateway™ pDEST™8 Vector

Citations & References (5)

Invitrogen™

Gateway™ pDEST™8 Vector

お客様のあらゆる発現ニーズに対応するため、Invitrogenは大腸菌、昆虫、酵母、哺乳類細胞での発現や、天然型タンパク質、N末端またはC末端融合タンパク質の産出に対応する最先端のGateway™デスティネーションベクターを提供しています。すべてのGateway™デスティネーションベクターには、エントリークローンかUltimate™ RFクローンかにかかわらず詳細を見る

製品番号（カタログ番号）	数量
11804010 または、製品番号11804-010	6 μg

製品番号（カタログ番号） 11804010

または、製品番号11804-010

価格（JPY）

68,100

お問い合わせください ›

お客様のあらゆる発現ニーズに対応するため、Invitrogenは大腸菌、昆虫、酵母、哺乳類細胞での発現や、天然型タンパク質、N末端またはC末端融合タンパク質の産出に対応する最先端のGateway™デスティネーションベクターを提供しています。すべてのGateway™デスティネーションベクターには、エントリークローンかUltimate™ RFクローンかにかかわらず、任意のatt Lサイドフラグメントでの組換えのためのatt R部位があります。以下の表に、使用可能な幅広いデスティネーションベクターを示します。

必要な追加の原材料、個別に使用可能：Gateway™エントリークローン、Gateway™ LRクロナーゼ™酵素ミックス、および反応バッファー。

研究用にのみ使用できます。診断用には使用いただけません。

製品タイプ発現ベクター

数量6 μg

ベクターpDEST、Gatewayバキュロウイルスベクター

クローニング法Gateway™

製品ラインGateway

プロモーターポリヘドリン

タンパク質タグタグなし

Unit SizeEach

組成および保存条件

すべてのデスティネーションベクターは凍結乾燥された超らせん型で提供されます。

よくあるご質問（FAQ）

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Gateway™ pDEST™8 Vector FAQs

引用および参考文献 (5)

引用および参考文献

Abstract

Target-induced formation of neuraminidase inhibitors from in vitro virtual combinatorial libraries.

Authors: HochgÃ¼rtel Matthias; Kroth Heiko; Piecha Dorothea; Hofmann Michael W; Nicolau Claude; Krause Sonja; Schaaf Otmar; Sonnenmoser Gabriele; Eliseev Alexey V;

Journal:Proc Natl Acad Sci U S A

PubMed ID:11891312

'Neuraminidase, a key enzyme responsible for influenza virus propagation, has been used as a template for selective synthesis of small subsets of its own inhibitors from theoretically highly diverse dynamic combinatorial libraries. We show that the library building blocks, aldehydes and amines, form significant amounts of the library components resulting ... More

Enhanced production of green fluorescent fusion proteins in a baculovirus expression system by addition of secretion signal.

Authors:Katagiri Y, Ingham KC.

Journal:Biotechniques

PubMed ID:12139250

All Six Modules of the Gelatin-binding Domain of Fibronectin Are Required for Full Affinity.

Authors:Katagiri Y, Brew SA, Ingham KC,

Journal:J Biol Chem

PubMed ID:12538576

The gelatin-binding sites of fibronectin are confined to a 42-kDa region having four type I and two type II modules in the following order: I(6)-II(1)-II(2)-I(7)-I(8)-I(9). To determine the relative importance of each module for recognition of gelatin, recombinant green fluorescent fusion proteins were prepared in which individual modules or groups ... More

Cleavage of von Willebrand Factor Requires the Spacer Domain of the Metalloprotease ADAMTS13.

Authors:Zheng X, Nishio K, Majerus EM, Sadler JE,

Journal:J Biol Chem

PubMed ID:12791682

ADAMTS13 consists of a reprolysin-type metalloprotease domain followed by a disintegrin domain, a thrombospondin type 1 motif (TSP1), Cys-rich and spacer domains, seven more TSP1 motifs, and two CUB domains. ADAMTS13 limits platelet accumulation in microvascular thrombi by cleaving the Tyr1605-Met1606 bond in von Willebrand factor, and ADAMTS13 deficiency causes ... More

DNA cloning using in vitro site-specific recombination.

Authors: Hartley J L; Temple G F; Brasch M A;

Journal:Genome Res

PubMed ID:11076863

As a result of numerous genome sequencing projects, large numbers of candidate open reading frames are being identified, many of which have no known function. Analysis of these genes typically involves the transfer of DNA segments into a variety of vector backgrounds for protein expression and functional analysis. We describe ... More