Geneticin™ Selective Antibiotic (G418 Sulfate), Powder, 1 g

Geneticin™ Selective Antibiotic (G418 Sulfate), Powder, 1 g - Citations

View additional product information for Geneticin™ Selective Antibiotic (G418 Sulfate), Powder - Citations (11811098, 11811031, 11811023)

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Abstract

Cloning and functional expression of a thyrotropin receptor cDNA from rat fat cells.

AuthorsEndo T; Ohta K; Haraguchi K; Onaya T;

JournalJ Biol Chem

PubMed ID7738021

Thyrotropin receptor (TSH-R) has been thought to be thyroid-specific, but, by Northern blot analysis, we found that rat adipose tissue expressed TSH-R mRNAs in amounts approaching those in the thyroid. To investigate the function of TSH-R from adipose tissue, we screened a rat fat cell lambda gt11 cDNA library for ... More

The connecting segment between both epidermal growth factor-like domains in blood coagulation factor IX contributes to stimulation by factor VIIIa and its isolated A2 domain.

AuthorsCelie Patrick H N; Van Stempvoort Gunny; Fribourg Caroline; Schurgers Leon J; Lenting Peter J; Mertens Koen;

JournalJ Biol Chem

PubMed ID11925427

The light chain of activated factor IX comprises multiple interactions between both epidermal growth factor-like domains that contribute to enzymatic activity and binding of factor IXa to its cofactor factor VIIIa. To investigate the association between factor IXa-specific properties and surface-exposed structure elements, chimeras were constructed in which the interconnection ... More

DBC2, a candidate for a tumor suppressor gene involved in breast cancer.

AuthorsHamaguchi Masaaki; Meth Jennifer L; von Klitzing Christine; Wei Wen; Esposito Diane; Rodgers Linda; Walsh Tom; Welcsh Piri; King Mary-Claire; Wigler Michael H;

JournalProc Natl Acad Sci U S A

PubMed ID12370419

A previously uncharacterized gene, DBC2 (deleted in breast cancer), was cloned from a homozygously deleted region at human chromosome 8p21. DBC2 contains a highly conserved RAS domain and two putative protein interacting domains. Our analyses indicate that DBC2 is the best candidate tumor suppressor gene from this region. It lies ... More

Role of tyrosine kinase Jak2 in prolactin-induced differentiation and growth of mammary epithelial cells.

AuthorsXie Jianwu; LeBaron Matthew J; Nevalainen Marja T; Rui Hallgeir;

JournalJ Biol Chem

PubMed ID11821424

Genetic studies in mice have established a critical role for prolactin receptors and transcription factor Stat5 in mammary gland differentiation. However, the enzymatic coupling between prolactin receptors and Stat5 in this process has not been established. In addition to Jak2, several other tyrosine kinases reportedly also are associated with prolactin ... More

A functional role for the B56 alpha-subunit of protein phosphatase 2A in ceramide-mediated regulation of Bcl2 phosphorylation status and function.

Authors Ruvolo Peter P; Clark Warren; Mumby Marc; Gao Fengqin; May W Stratford;

JournalJ Biol Chem

PubMed ID11929874

'Recently it has been shown that the potent apoptotic agent ceramide activates a mitochondrial protein phosphatase 2A (PP2A) and promotes dephosphorylation of the anti-apoptotic molecule Bcl2 (Ruvolo, P. P., Deng, X., Ito, T., Carr, B. K., and May, W. S. (1999) J. Biol. Chem. 274, 20296-20300). In cells expressing Bcl2, ... More

Targeted gene disruption reveals the role of cysteinyl leukotriene 1 receptor in the enhanced vascular permeability of mice undergoing acute inflammatory responses.

Authors Maekawa Akiko; Austen K Frank; Kanaoka Yoshihide;

JournalJ Biol Chem

PubMed ID11932261

'The cysteinyl leukotrienes (cysLTs), leukotriene (LT) C(4), LTD(4), and LTE(4), are proinflammatory lipid mediators generated in the mouse by hematopoietic cells such as macrophages and mast cells. There are two mouse receptors for the cysLTs, CysLT(1) receptor (CysLT(1)R) and CysLT(2)R, which are 38% homologous and are located on mouse chromosomes ... More

Residues throughout the cytoplasmic domain affect the internalization efficiency of P-selectin.

Authors Setiadi H; Disdier M; Green S A; Canfield W M; McEver R P;

JournalJ Biol Chem

PubMed ID7592923

'The cytoplasmic domains of many membrane proteins have short sequences, usually including a tyrosine or a di-leucine, that function as sorting signals. P-selectin is an adhesion receptor for leukocytes that is expressed on activated platelets and endothelial cells. Its 35-residue cytoplasmic domain contains signals for sorting into regulated secretory granules, ... More

IRE1-mediated unconventional mRNA splicing and S2P-mediated ATF6 cleavage merge to regulate XBP1 in signaling the unfolded protein response.

Authors Lee Kyungho; Tirasophon Witoon; Shen Xiaohua; Michalak Marek; Prywes Ron; Okada Tetsuya; Yoshida Hiderou; Mori Kazutoshi; Kaufman Randal J;

JournalGenes Dev

PubMed ID11850408

'All eukaryotic cells respond to the accumulation of unfolded proteins in the endoplasmic reticulum (ER) by signaling an adaptive pathway termed the unfolded protein response (UPR). In yeast, a type-I ER transmembrane protein kinase, Ire1p, is the proximal sensor of unfolded proteins in the ER lumen that initiates an unconventional ... More

Functional coupling between various phospholipase A2s and cyclooxygenases in immediate and delayed prostanoid biosynthetic pathways.

Authors Murakami M; Kambe T; Shimbara S; Kudo I;

JournalJ Biol Chem

PubMed ID9915849

'Several distinct phospholipase A2s (PLA2s) and two cyclooxygenases (COXs) were transfected, alone or in combination, into human embryonic kidney 293 cells, and their functional coupling during immediate and delayed prostaglandin (PG)-biosynthetic responses was reconstituted. Signaling PLA2s, i.e. cytosolic PLA2 (cPLA2) (type IV) and two secretory PLA2s (sPLA2), types IIA (sPLA2-IIA) ... More

Hormone-sensitive lipase deficiency in mice causes diglyceride accumulation in adipose tissue, muscle, and testis.

Authors Haemmerle Guenter; Zimmermann Robert; Hayn Marianne; Theussl Christian; Waeg Georg; Wagner Elke; Sattler Wolfgang; Magin Thomas M; Wagner Erwin F; Zechner Rudolf;

JournalJ Biol Chem

PubMed ID11717312

'Hormone-sensitive lipase (HSL) is expressed predominantly in white and brown adipose tissue where it is believed to play a crucial role in the lipolysis of stored triglycerides (TG), thereby providing the body with energy substrate in the form of free fatty acids (FFA). From in vitro assays, HSL is known ... More

Regulation in vivo of the growth of Leydig cell tumors by antisense ribonucleic acid for parathyroid hormone-related peptide.

Authors Rabbani S A; Gladu J; Liu B; Goltzman D;

JournalEndocrinology

PubMed ID7588290

'PTH-related peptide (PTHrP) has been shown to be the major mediator of hypercalcemia of malignancy, but may also exert effects on cell growth and differentiation. The Leydig cell tumor H-500, when implanted in Fischer rats, produces abundant PTHrP and eventually causes the death of the host animal. In the present ... More

alpha-Synuclein protects against oxidative stress via inactivation of the c-Jun N-terminal kinase stress-signaling pathway in neuronal cells.

Authors Hashimoto Makoto; Hsu Leigh J; Rockenstein Edward; Takenouchi Takato; Mallory Margaret; Masliah Eliezer;

JournalJ Biol Chem

PubMed ID11790792

'The expression of alpha-synuclein, a synaptic molecule implicated in the pathogenesis of neurodegenerative disorders such as Parkinson''s disease and Lewy body disease is increased upon injury to the nervous system, indicating that it might play a role in regeneration and plasticity; however, the mechanisms are unclear. Because c-Jun N-terminal kinase ... More

Structural basis of G protein specificity of human endothelin receptors. A study with endothelinA/B chimeras.

Authors Takagi Y; Ninomiya H; Sakamoto A; Miwa S; Masaki T;

JournalJ Biol Chem

PubMed ID7730310

'The endothelin (ET) family of peptides acts via two subtypes of guanine nucleotide-binding regulatory protein (G protein)-coupled receptors termed ETA and ETB. ET-1 stimulated cAMP formation in Chinese hamster ovary (CHO) cells stably expressing human wild-type ETA (CHO/hETA cells) while it inhibited cAMP formation in CHO cells expressing human wild-type ... More

Cholesteryl ester is transported from caveolae to internal membranes as part of a caveolin-annexin II lipid-protein complex.

Authors Uittenbogaard Annette; Everson William V; Matveev Sergey V; Smart Eric J;

JournalJ Biol Chem

PubMed ID11733519

'We previously demonstrated that in Chinese hamster ovary cells scavenger receptor, class B, type I-dependent selective cholesteryl ester uptake occurs in caveolae. In the present study we hypothesized that cholesteryl ester is transported from caveolae through the cytosol to an internal membrane by a caveolin chaperone complex similar to the ... More

Biosynthetic processing of the pro-alpha 1(V)2pro-alpha 2(V) collagen heterotrimer by bone morphogenetic protein-1 and furin-like proprotein convertases.

Authors UnsÃ¶ld Christine; Pappano William N; Imamura Yasutada; Steiglitz Barry M; Greenspan Daniel S;

JournalJ Biol Chem

PubMed ID11741999

'The low abundance fibrillar collagen type V is incorporated into and regulates the diameters of type I collagen fibrils. Bone morphogenetic protein-1 (BMP-1) is a metalloprotease that plays key roles in regulating formation of vertebrate extracellular matrix; it cleaves the C-propeptides of the major fibrillar procollagens I-III and processes precursors ... More

Dopamine beta-monooxygenase signal/anchor sequence alters trafficking of peptidylglycine alpha-hydroxylating monooxygenase.

Authors Oyarce A M; Steveson T C; Jin L; Eipper B A;

JournalJ Biol Chem

PubMed ID11418593

'Dopamine beta-monooxygenase (DBM) and peptidylglycine alpha-hydroxylating monooxygenase (PHM) are essential for the biosynthesis of catecholamines and amidated peptides, respectively. The enzymes share a conserved catalytic core. We studied the role of the DBM signal sequence by appending it to soluble PHM (PHMs) and expressing the DBMsignal/PHMs chimera in AtT-20 and ... More

Core protein dependence of epimerization of glucuronosyl residues in galactosaminoglycans.

Authors Seidler Daniela G; Breuer Egon; Grande-Allen K Jane; Hascall Vincent C; Kresse Hans;

JournalJ Biol Chem

PubMed ID12207034

'Chondroitin sulfate and dermatan sulfate proteoglycans are distinguished by differences in their proportion of d-glucuronosyl and l-iduronosyl residues, the latter being formed by chondroitin-glucuronate 5-epimerase during or after glycosaminoglycan chain polymerization. To investigate the influence of the core protein on the extent of epimerization, we expressed chimeric proteins in 293 ... More

An alternative processing of integrin alpha(v) subunit in tumor cells by membrane type-1 matrix metalloproteinase.

Authors Ratnikov Boris I; Rozanov Dmitri V; Postnova Tanya I; Baciu Peter G; Zhang Heying; DiScipio Richard G; Chestukhina Galina G; Smith Jeffrey W; Deryugina Elena I; Strongin Alex Y;

JournalJ Biol Chem

PubMed ID11741954

'Membrane type-1 matrix metalloproteinase (MT1-MMP) and alpha(v)beta(3) integrin are both essential to cell invasion. Maturation of integrin pro-alpha(v)chain (pro-alpha(v)) involves its cleavage by proprotein convertases (PC) to form the disulfide-bonded 125-kDa heavy and 25-kDa light alpha chains. Our report presents evidence of an alternative pathway of pro-alpha(v) processing involving MT1-MMP. ... More

Cooperative regulation of the invasive and metastatic phenotypes by different domains of the type I insulin-like growth factor receptor beta subunit.

Authors Brodt P; Fallavollita L; Khatib A M; Samani A A; Zhang D;

JournalJ Biol Chem

PubMed ID11445567

'The receptor for the type 1 insulin-like growth factor (IGF-I) regulates multiple cellular functions impacting on the metastatic phenotype of tumor cells, including cellular proliferation, anchorage-independent growth, survival, migration, synthesis of the 72-kDa type IV collagenase and invasion. We have used site-directed mutagenesis to generate domain-specific mutants of the receptor ... More

Human mitochondrial 5'-deoxyribonucleotidase. Overproduction in cultured cells and functional aspects.

Authors Gallinaro Lisa; Crovatto Katia; Rampazzo Chiara; Pontarin Giovanna; Ferraro Paola; Milanesi Eva; Reichard Peter; Bianchi Vera;

JournalJ Biol Chem

PubMed ID12124385

'Deoxynucleoside triphosphates (dNTPs) used for mitochondrial DNA replication are mainly formed by phosphorylation of deoxynucleosides imported into mitochondria from the cytosol. We earlier obtained evidence for a mitochondrial 5''-nucleotidase (dNT2) with a pronounced specificity for dUMP and dTMP and suggested that the enzyme protects mitochondrial DNA replication from excess dTTP. ... More

beta 1-Integrin-mediated Glioma Cell Adhesion and Free Radical-induced Apoptosis Are Regulated by Binding to a C-terminal Domain of PG-M/Versican.

Authors Wu Yaojiong; Chen Liwen; Zheng Peng-Sheng; Yang Burton B;

JournalJ Biol Chem

PubMed ID11805102

'Integrins are cell-surface glycoproteins that mediate cell activities, including tissue morphogenesis, development, immune response, and cancer, through interaction with extracellular proteins. Here we report a novel means by which integrin signaling and functions are regulated. In pull-down assays and immunoprecipitation, beta(1)-integrin bound to the C-terminal domain of PG-M/versican, an extracellular ... More

Smac agonists sensitize for Apo2L/TRAIL- or anticancer drug-induced apoptosis and induce regression of malignant glioma in vivo.

Authors Fulda Simone; Wick Wolfgang; Weller Michael; Debatin Klaus-Michael;

JournalNat Med

PubMed ID12118245

'A major concern in cancer therapy is resistance of tumors such as glioblastoma to current treatment protocols. Here, we report that transfer of the gene encoding second mitochondria-derived activator of caspase (Smac) or Smac peptides sensitized various tumor cells in vitro and malignant glioma cells in vivo for apoptosis induced ... More

Inhibition of phosphorylation of BAD and Raf-1 by Akt sensitizes human ovarian cancer cells to paclitaxel.

Authors Mabuchi Seiji; Ohmichi Masahide; Kimura Akiko; Hisamoto Koji; Hayakawa Jun; Nishio Yukihiro; Adachi Kazushige; Takahashi Kazuhiro; Arimoto-Ishida Emi; Nakatsuji Yuki; Tasaka Keiichi; Murata Yuji;

JournalJ Biol Chem

PubMed ID12087097

'We studied the roles of the phosphatidylinositol 3-kinase (PI-3K)-Akt-BAD cascade, ERK-BAD cascade, and Akt-Raf-1 cascade in the paclitaxel-resistant SW626 human ovarian cancer cell line, which lacks functional p53. Treatment of SW626 cells with paclitaxel activates Akt and ERK with different time frames. Interference with the Akt cascade either by treatment ... More

Targeted disruption of the MKK4 gene causes embryonic death, inhibition of c-Jun NH2-terminal kinase activation, and defects in AP-1 transcriptional activity.

AuthorsYang D, Tournier C, Wysk M, Lu HT, Xu J, Davis RJ, Flavell RA,

JournalProc Natl Acad Sci U S A

PubMed ID9096336

'MKK4 is a member of the mitogen-activated protein kinase kinase group of dual specificity protein kinases that functions as an activator of the c-Jun NH2-terminal kinase (JNK) in vitro. To examine the function of MKK4 in vivo, we investigated the effect of targeted disruption of the MKK4 gene. Crosses of ... More

Costimulation of T cell activation by integrin-associated protein (CD47) is an adhesion-dependent, CD28-independent signaling pathway.

AuthorsReinhold MI, Lindberg FP, Kersh GJ, Allen PM, Brown EJ

JournalJ Exp Med

PubMed ID8996237

'The integrin-associated protein (IAP, CD47) is a 50-kD plasma membrane protein with a single extracellular immunoglobulin variable (IgV)-like domain, a multiply membrane-spanning segment, and alternatively spliced short cytoplasmic tails. On neutrophils, IAP has been shown to function in a signaling complex with beta 3 integrins. However, the function of IAP ... More

Ligand binding to macrophage scavenger receptor-A induces urokinase- type plasminogen activator expression by a protein kinase-dependent signaling pathway.

AuthorsHsu HY, Hajjar DP, Khan KM, Falcone DJ

JournalJ Biol Chem

PubMed ID9422792

'Macrophage scavenger receptor-type A (MSR-A) has been implicated in the transmission of cell signals and the regulation of diverse cellular functions (Falcone, D. J., and Ferenc, M. J. (1988) J. Cell. Physiol. 135, 387-396; Falcone, D. J., McCaffrey, T. A., and Vergilio, J. A. (1991) J. Biol. Chem. 266, 22726-22732; ... More

Cellular response to oncogenic ras involves induction of the Cdk4 and Cdk6 inhibitor p15(INK4b).

AuthorsMalumbres M, Perez De Castro I, Hernandez MI, Jimenez M, Corral T, Pellicer A

JournalMol Cell Biol

PubMed ID10733595

'The cell cycle inhibitor p15(INK4b) is frequently inactivated by homozygous deletion together with p16(INK4a) and p19(ARF) in some types of tumors. Although the tumor suppressor capability of p15(INK4b) is still questioned, it has been found to be specifically inactivated by hypermethylation in hematopoietic malignancies in the absence of p16(INK4a) alterations. ... More

Proteolytic activation of protein kinase C-epsilon by caspase-mediated processing and transduction of antiapoptotic signals.

AuthorsBasu A, Lu D, Sun B, Moor AN, Akkaraju GR, Huang J,

JournalJ Biol Chem

PubMed ID12198125

'Several novel protein kinase C (PKC) isozymes have been identified as substrates for caspase-3. We have previously shown that novel PKCepsilon is cleaved during apoptosis in MCF-7 cells that lack any functional caspase-3. In the present study, we show that in vitro-translated PKCepsilon is processed by human recombinant caspase-3, -7, ... More

Molecular cloning and characterization of a novel UDP-Gal:GalNAc(alpha) peptide beta 1,3-galactosyltransferase (C1Gal-T2), an enzyme synthesizing a core 1 structure of O-glycan.

AuthorsKudo T, Iwai T, Kubota T, Iwasaki H, Takayma Y, Hiruma T, Inaba N, Zhang Y, Gotoh M, Togayachi A, Narimatsu H,

JournalJ Biol Chem

PubMed ID12361956

'Recently, a UDP-Gal:GalNAc(alpha) peptide beta1,3-galactosyltransferase (core 1 synthase 1; C1Gal-T1) has been purified from rat liver and its complementary DNA cloned from several species. We isolated a second candidate for core 1 synthase from a Colo205 cDNA library and named it C1Gal-T2. The deduced amino acid sequence of C1Gal-T2, having ... More

Alternative Splicing of the Adenylyl Cyclase Stimulatory G-protein Galpha s Is Regulated by SF2/ASF and Heterogeneous Nuclear Ribonucleoprotein A1 (hnRNPA1) and Involves the Use of an Unusual TG 3'-Splice Site.

Authors Pollard Alison J; Krainer Adrian R; Robson Stephen C; Europe-Finner G Nicholas;

JournalJ Biol Chem

PubMed ID11825891

'The factors involved in regulating alternative splicing of the human adenylyl cyclase stimulatory G-protein Galpha(s) in different cell types remain undefined. We have designed a Galpha(s) minigene that retains the signals required for Galpha(s) alternative splicing in vivo. Employing transient transfection of human myometrial smooth muscle cells and HeLa cells, ... More

A region of human CD14 required for lipopolysaccharide binding.

Authors Viriyakosol S; Kirkland T N;

JournalJ Biol Chem

PubMed ID7529231

'CD14, a glycosylphosphatidylinositol-anchored protein on the surface of monocytes, macrophages, and polymorphonuclear leukocytes, is a receptor for lipopolysaccharide (LPS). CD14 binding of LPS is enhanced by serum proteins, especially lipopolysaccharide binding protein. The serum-dependent binding of LPS to CD14 stimulates macrophages to make cytokines, which can cause septic shock in ... More

Analysis of the hormone-dependent regulation of a JunD-estrogen receptor chimera.

Authors Francis M K; Phinney D G; Ryder K;

JournalJ Biol Chem

PubMed ID7744790

'The modular ligand-binding domains of steroid receptors have been widely used to generate protein chimeras that are ligand dependent for activity. In a similar manner, we generated a series of conditionally active JunD and c-Fos proteins by fusing their carboxyl (COOH)-terminal ends with a COOH-terminal fragment of the human estrogen ... More

Dimerization of a selectin and its ligand stabilizes cell rolling and enhances tether strength in shear flow.

Authors Ramachandran V; Yago T; Epperson T K; Kobzdej M M; Nollert M U; Cummings R D; Zhu C; McEver R P;

JournalProc Natl Acad Sci U S A

PubMed ID11526223

'Selectins mediate rolling of leukocytes by rapid formation and dissociation of selectin-ligand bonds, which are assumed to require high mechanical strength to prevent premature dissociation by the forces applied in shear flow. This assumption is based largely on the observation that increasing wall shear stress increases only modestly the dissociation ... More

PPARgamma knockdown by engineered transcription factors: exogenous PPARgamma2 but not PPARgamma1 reactivates adipogenesis.

Authors Ren Delin; Collingwood Trevor N; Rebar Edward J; Wolffe Alan P; Camp Heidi S;

JournalGenes Dev

PubMed ID11782439

'To determine functional differences between the two splice variants of PPARgamma (gamma1 and gamma2), we sought to selectively repress gamma2 expression by targeting engineered zinc finger repressor proteins (ZFPs) to the gamma2-specific promoter, P2. In 3T3-L1 cells, expression of ZFP55 resulted in >50% reduction in gamma2 expression but had no ... More

Functional role of arginine 373 in substrate translocation by the reduced folate carrier.

AuthorsSadlish H, Williams FM, Flintoff WF,

JournalJ Biol Chem

PubMed ID12194981

'The reduced folate carrier (RFC) plays a critical role in the cellular uptake of folates. However, little is known regarding the mechanism used to transport substrates or the tertiary structure of the protein. Through the analysis of a Chinese hamster ovary cell line deficient in folate uptake, we have identified ... More

The human organic cation transporter (hOCT2) recognizes the degree of substrate ionization.

Authors Barendt Wendy M; Wright Stephen H;

JournalJ Biol Chem

PubMed ID11953440

'The organic cation transporter, OCT2, plays a role in renal secretion of a broad array of weak bases. To determine whether the degree of ionization of these compounds plays a role in their interaction with OCT2, we examined the influence of external pH values on the activity of the human ... More

Contribution of estrogen receptor alpha to oncogenic K-Ras-mediated NIH3T3 cell transformation and its implication for escape from senescence by modulating the p53 pathway.

Authors Kato Kiyoko; Horiuchi Shinji; Takahashi Akira; Ueoka Yousuke; Arima Takahiro; Matsuda Takao; Kato Hidenori; Nishida Ji Jun-ichi; Nakabeppu Yusaku; Wake Norio;

JournalJ Biol Chem

PubMed ID11781307

'We previously reported that enhanced transcriptional activation of estrogen receptor alpha (ERalpha) contributed to [(12)Val]K-Ras-mediated NIH3T3 cell transformation. Functional inactivation of ERalpha by a dominant negative mutant of ERalpha (DNER) in the presence of activated K-Ras 4B mutant arrested the cell cycle at G(0)/G(1), subsequently provoking replicative cell senescence, finally ... More

Gap junctional intercellular communication contributes to hormonal responsiveness in osteoblastic networks.

Authors Vander Molen M A; Rubin C T; McLeod K J; McCauley L K; Donahue H J;

JournalJ Biol Chem

PubMed ID8647809

'To evaluate whether intercellular coupling via connexin43 gap junction channels modulates hormonal responsiveness of cells in contact, we have created osteoblastic cell lines deficient in connexin43. Osteoblastic ROS 17/2.8 cells were transfected with a plasmid containing an antisense cDNA construct to rat connexin43. Control transfection did not alter cell-to-cell coupling ... More

Steroid ligands bind human sex hormone-binding globulin in specific orientations and produce distinct changes in protein conformation.

Authors Grishkovskaya Irina; Avvakumov George V; Hammond Geoffrey L; Catalano Maria G; Muller Yves A;

JournalJ Biol Chem

PubMed ID12065592

'The amino-terminal laminin G-like domain of human sex hormone-binding globulin (SHBG) contains a single high affinity steroid-binding site. Crystal structures of this domain in complex with several different steroid ligands have revealed that estradiol occupies the SHBG steroid-binding site in an opposite orientation when compared with 5 alpha-dihydrotestosterone or C19 ... More

Activation and inhibition of G protein-coupled inwardly rectifying potassium (Kir3) channels by G protein beta gamma subunits.

AuthorsLei Q, Jones MB, Talley EM, Schrier AD, McIntire WE, Garrison JC, Bayliss DA

JournalProc Natl Acad Sci U S A

PubMed ID10944236

'G protein-coupled inwardly rectifying potassium (GIRK) channels can be activated or inhibited by different classes of receptors, suggesting a role for G proteins in determining signaling specificity. Because G protein betagamma subunits containing either beta1 or beta2 with multiple Ggamma subunits activate GIRK channels, we hypothesized that specificity might be ... More

Distinct residues in the carboxyl tail mediate agonist-induced desensitization and internalization of the human dopamine D1 receptor.

Authors Lamey Michael; Thompson Miles; Varghese George; Chi Hong; Sawzdargo Marek; George Susan R; O'Dowd Brian F;

JournalJ Biol Chem

PubMed ID11773080

'We have shown in a previous study that desensitization and internalization of the human dopamine D(1) receptor following short-term agonist exposure are mediated by temporally and biochemically distinct mechanisms. In the present study, we have used site-directed mutagenesis to remove potential phosphorylation sites in the third intracellular loop and carboxyl ... More

Activation of the proteolytic activity of ADAMTS4 (aggrecanase-1) by C-terminal truncation.

Authors Gao Gui; Westling Jennifer; Thompson Vivian P; Howell Troy D; Gottschall Paul E; Sandy John D;

JournalJ Biol Chem

PubMed ID11796708

'Proteolysis of the hyalectans (aggrecan, versican, brevican) in vivo appears to result from the activity of ADAMTS4 (aggrecanase-1, herein referred to as an hyalectanase). To examine the mode of activation of ADAMTS4, a human chondrosarcoma cell line, JJ012, has been stably transfected with the full-length c-DNA for human ADAMTS4. The ... More

The transcription factor GATA-3 is necessary and sufficient for Th2 cytokine gene expression in CD4 T cells.

AuthorsZheng W, Flavell RA

JournalCell

PubMed ID9160750

'CD4 T cells potentiate the inflammatory or humoral immune response through the action of Th1 and Th2 cells, respectively. The molecular basis of the differentiation of these cells from naive T cell precursors is, however, unclear. We found that GATA-3 was selectively expressed in Th2 cells. GATA-3 is expressed at ... More

Induction of apoptosis by pyrrolidinedithiocarbamate and N-acetylcysteine in vascular smooth muscle cells.

Authors Tsai J C; Jain M; Hsieh C M; Lee W S; Yoshizumi M; Patterson C; Perrella M A; Cooke C; Wang H; Haber E; Schlegel R; Lee M E;

JournalJ Biol Chem

PubMed ID8631978

'Pyrrolidinedithiocarbamate (PDTC) and N-acetylcysteine (NAC) have been used as antioxidants to prevent apoptosis in lymphocytes, neurons, and vascular endothelial cells. We report here that PDTC and NAC induce apoptosis in rat and human smooth muscle cells. In rat aortic smooth muscle cells, PDTC induced cell shrinkage, chromatin condensation, and DNA ... More

Constitutive Internalization of Constitutively Active Angiotensin II AT1A Receptor Mutants Is Blocked by Inverse Agonists.

Authors Miserey-Lenkei Stephanie; Parnot Charles; Bardin Sabine; Corvol Pierre; Clauser Eric;

JournalJ Biol Chem

PubMed ID11729186

'As constitutively active mutants (CAMs) mimic an active conformation, they can be used to characterize the process of G protein-coupled receptor activation. Here, we used CAMs to study the link between activation and internalization of the angiotensin II AT(1A) receptor. The cellular localization of fluorescently tagged N111A, I245T, and L305Q ... More

Signaling through the Smad pathway by insulin-like growth factor-binding protein-3 in breast cancer cells. Relationship to transforming growth factor-beta 1 signaling.

Authors Fanayan Susan; Firth Sue M; Baxter Robert C;

JournalJ Biol Chem

PubMed ID11751851

'We previously demonstrated in T47D cells transfected to express the transforming growth factor-beta receptor type II (TGF-betaRII) that insulin-like growth factor binding protein-3 (IGFBP-3) could stimulate Smad2 and Smad3 phosphorylation, potentiate TGF-beta1-stimulated Smad phosphorylation, and cooperate with exogenous TGF-beta1 in cell growth inhibition (Fanayan, S., Firth, S. M., Butt, A. ... More

Galectin-3 phosphorylation is required for its anti-apoptotic function and cell cycle arrest.

Authors Yoshii Tadashi; Fukumori Tomoharu; Honjo Yuichiro; Inohara Hidenori; Kim Hyeong-Reh Choi; Raz Avraham;

JournalJ Biol Chem

PubMed ID11724777

'Galectin-3, a beta-galactoside-binding protein, is implicated in cell growth, adhesion, differentiation, and tumor progression by interactions with its ligands. Recent studies have revealed that galectin-3 suppresses apoptosis and anoikis that contribute to cell survival during metastatic cascades. Previously, it has been shown that human galectin-3 undergoes post-translational signaling modification of ... More

BCL2 regulates neural differentiation.

Authors Zhang K Z; Westberg J A; HÃ¶lttÃ¤ E; Andersson L C;

JournalProc Natl Acad Sci U S A

PubMed ID8633098

'A main function attributed to the BCL2 protein is its ability to confer resistance against apoptosis. In addition to the constitutively high expression of BCL2, caused by gene rearrangement in follicular lymphomas, elevated expression of the BCL2 gene has been found in differentiating hematopoietic, neural, and epithelial tissues. To address ... More

Regulation of neural differentiation by normal and mutant (G654A, amyloidogenic) gelsolin.

Authors Westberg J A; Zhang K Z; Andersson L C;

JournalFASEB J

PubMed ID10463954

'Gelsolin belongs to a family of proteins that modulate the structural dynamics of cytoskeletal actin. Gelsolin activity is required for the redistribution of actin occurring during membrane ruffling, cell crawling, and platelet activation. A point mutation (G654A) in the gelsolin gene causes a dominantly inherited systemic amyloidosis called familial amyloidosis ... More

Endothelial cell-specific knockout of connexin 43 causes hypotension and bradycardia in mice.

Authors Liao Y; Day K H; Damon D N; Duling B R;

JournalProc Natl Acad Sci U S A

PubMed ID11481448

Connexin 43 (Cx43) is a protein expressed in a variety of mammalian tissues. However, the lack of specific blockers and the absence of known genetic mutants have hampered the investigation of the function of this protein. Cx43-null mice die shortly after birth, thus preventing functional studies in vivo. Here, we ... More

Missense mutations interfere with VEGFR-3 signalling in primary lymphoedema.

AuthorsKarkkainen MJ, Ferrell RE, Lawrence EC, Kimak MA, Levinson KL, McTigue MA, Alitalo K, Finegold DN

JournalNature Genetics

PubMed ID10835628

Primary lymphoedema is a rare, autosomal dominant disorder that leads to a disabling and disfiguring swelling of the extremities and, when untreated, tends to worsen with time. Here we link primary human lymphoedema to the FLT4 locus, encoding vascular endothelial growth factor receptor-3 (VEGFR-3), in several families. All disease-associated alleles ... More

Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells.

Authors Zacharias David A; Violin Jonathan D; Newton Alexandra C; Tsien Roger Y;

JournalScience

PubMed ID11988557

Many proteins associated with the plasma membrane are known to partition into submicroscopic sphingolipid- and cholesterol-rich domains called lipid rafts, but the determinants dictating this segregation of proteins in the membrane are poorly understood. We suppressed the tendency of Aequorea fluorescent proteins to dimerize and targeted these variants to the ... More

Functional characterization of ProSAAS: similarities and differences with 7B2.

Authors Fortenberry Yolanda; Hwang Jae-Ryoung; Apletalina Ekaterina V; Lindberg Iris;

JournalJ Biol Chem

PubMed ID11719503

Prohormone convertases (PC) 1 and 2, enzymes found primarily in neuroendocrine tissues, are thought to mediate the proteolytic cleavage of many peptide precursors. To date, endogenous binding proteins for both PC2 (7B2) and PC1 (proSAAS) have been identified. Although 7B2 represents a potent inhibitor of PC2, the most important function ... More

Activation of c-Jun N-terminal kinase antagonizes an anti-apoptotic action of Bcl-2.

AuthorsPark J, Kim I, Oh YJ, Lee K, Han PL, Choi EJ

JournalJ Biol Chem

PubMed ID9201973

Bcl-2 is an intracellular membrane-associated protein that prevents cell death induced by a variety of apoptotic stimuli. A mechanism by which Bcl-2 exerts an anti-cell death effect is, however, not fully understood. In the present study, Bcl-2 suppressed cell death of N18TG neuroglioma cells caused by various apoptotic stresses, including ... More

Tyrosine sulfation of the amino terminus of CCR5 facilitates HIV-1 entry.

AuthorsFarzan M, Mirzabekov T, Kolchinsky P, Wyatt R, Cayabyab M, Gerard NP, Gerard C, Sodroski J, Choe H

JournalCell

PubMed ID10089882

Chemokine receptors and related seven-transmembrane-segment (7TMS) receptors serve as coreceptors for entry of human and simian immunodeficiency viruses (HIV-1, HIV-2, and SIV) into target cells. Each of these otherwise diverse coreceptors contains an N-terminal region that is acidic and tyrosine rich. Here, we show that the chemokine receptor CCR5, a ... More

Polarization of myosin II heavy chain-protein kinase C in chemotaxing dictyostelium cells.

AuthorsRubin H, Ravid S,

JournalJ Biol Chem

PubMed ID12130648

Eukaryotic cells need morphological polarity to carry out chemotaxis (Parent, C. A., Blacklock, B. J., Froehlich, W. M., Murphy, D. B., and Devreotes, P. N. (1998) Cell 95, 81-91; Jin, T., Zhang, N., Long, Y., Parent, C., and Devreotes, P. N. (2000) Science 287, 1034-1036; Servant, G., Weiner, O. D., ... More

The presenilin 2 mutation (N141I) linked to familial Alzheimer disease (Volga German families) increases the secretion of amyloid beta protein ending at the 42nd (or 43rd) residue [see comments]

AuthorsTomita T, Maruyama K, Saido TC, Kume H, Shinozaki K, Tokuhiro S, Capell A, Walter J, Grunberg J, Haass C, Iwatsubo T, Obata K

JournalProc Natl Acad Sci U S A

PubMed ID9050898

To gain insights into the significance of presenilins (PS) in the pathogenetic mechanisms of early-onset familial Alzheimer disease (FAD), we expressed cDNAs for wild-type PS2 and PS2 with the Volga German (N141I) mutation in cultured cells and then examined the metabolism of the transfected proteins and their effect on the ... More

Identification of CCR8: a human monocyte and thymus receptor for the CC chemokine I-309

AuthorsTiffany HL, Lautens LL, Gao JL, Pease J, Locati M, Combadiere C, Modi W, Bonner TI, Murphy PM

JournalJ Exp Med

PubMed ID9207005

The human CC chemokine I-309 is a potent monocyte chemoattractant and inhibits apoptosis in thymic cell lines. Here, we identify a specific human I-309 receptor, and name it CCR8 according to an accepted nomenclature system. The receptor has seven predicted transmembrane domains, is expressed constitutively in monocytes and thymus, and ... More

Human CD4+ cells transfected with IL-16 cDNA are resistant to HIV-1 infection: inhibition of mRNA expression [see comments]

AuthorsZhou P, Goldstein S, Devadas K, Tewari D, Notkins AL

JournalNat Med

PubMed ID9176493

Interleukin-16 (IL-16) is secreted by activated CD8+ T lymphocytes and acts on CD4+ T lymphocytes, monocytes and eosinophils. Recently, the C- terminal 130-amino acid portion of IL-16 was shown to suppress HIV-1 replication in vitro. To explore the potential of human IL-16 for gene therapy, this portion was transfected into ... More

Post-translational modifications of recombinant P-selectin glycoprotein ligand-1 required for binding to P- and E-selectin.

AuthorsLi F, Wilkins PP, Crawley S, Weinstein J, Cummings RD, McEver RP

JournalJ Biol Chem

PubMed ID8621728

P-selectin glycoprotein ligand-1 (PSGL-1) is a mucin-like ligand for P- and E-selectin on human leukocytes. PSGL-1 requires sialylated, fucosylated O-linked glycans and tyrosine sulfate to bind P-selectin. Less is known about the determinants that PSGL-1 requires to bind E- selectin. To further define the modifications required for PSGL-1 to bind ... More

Loss of E-cadherin-dependent cell-cell adhesion due to mutation of the beta-catenin gene in a human cancer cell line, HSC-39.

AuthorsKawanishi J, Kato J, Sasaki K, Fujii S, Watanabe N, Niitsu Y

JournalMol Cell Biol

PubMed ID7862112

Detachment of cell-cell adhesion is indispensable for the first step of invasion and metastasis of cancer. This mechanism is frequently associated with the impairment of either E-cadherin expression or function. However, mechanisms of such abnormalities have not been fully elucidated. In this study, we demonstrated that the function of E- ... More

The varicella-zoster virus immediate early protein, IE62, can positively regulate its cognate promoter.

AuthorsPerera LP, Mosca JD, Sadeghi-Zadeh M, Ruyechan WT, Hay J,

JournalVirology

PubMed ID1329324

Varicella-Zoster virus (VZV) is a neurotropic alphaherpes virus closely related to herpes simplex virus (HSV). However, unlike its close relative HSV, VZV lacks a functional alpha-TIF (alpha-gene transinducing factor) that activates the transcription of immediate early genes during the initial events of the virus life cycle. Hence, in the absence ... More

Formation of heteromeric gap junction channels by connexins 40 and 43 in vascular smooth muscle cells.

AuthorsHe DS, Jiang JX, Taffet SM, Burt JM

JournalProc Natl Acad Sci U S A

PubMed ID10339616

Connexin (Cx) 43 and Cx40 are coexpressed in several tissues, including cardiac atrial and ventricular myocytes and vascular smooth muscle. It has been shown that these Cxs form homomeric/homotypic channels with distinct permeability and gating properties but do not form functional homomeric/heterotypic channels. If these Cxs were to form heteromeric ... More

Discrimination between RelA and RelB transcriptional regulation by a dominant negative mutant of IkappaBalpha.

AuthorsFerreira V, Tarantino N, Korner M

JournalJ Biol Chem

PubMed ID9417120

RelA and RelB belong to the nuclear factor-kappaB (NF-kappaB-Rel) transcription factor family. Both proteins are structurally and functionally related, but their intracellular and tissue distributions are different. In resting cells, RelB is found mostly in the nucleus, whereas RelA is sequestered in the cytosol by protein inhibitors, among which IkappaBalpha ... More

Targeting of HIV-1 antigens for rapid intracellular degradation enhances cytotoxic T lymphocyte (CTL) recognition and the induction of de novo CTL responses in vivo after immunization.

AuthorsTobery TW, Siliciano RF

JournalJ Exp Med

PubMed ID9120397

CD8+ cytotoxic T lymphocytes (CTLs) have the ability to recognize and eliminate virally infected cells before new virions are produced within that cell. Therefore, a rapid and vigorous CD8+ CTL response, induced by vaccination, can, in principle, prevent disseminated infection in vaccinated individuals who are exposed to the relevant virus. ... More

Human macrophage-derived chemokine (MDC), a novel chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer cells.

AuthorsGodiska R, Chantry D, Raport CJ, Sozzani S, Allavena P, Leviten D, Mantovani A, Gray PW

JournalJ Exp Med

PubMed ID9151897

A cDNA encoding a novel human chemokine was isolated by random sequencing of cDNA clones from human monocyte-derived macrophages. This protein has been termed macrophage-derived chemokine (MDC) because it appears to be synthesized specifically by cells of the macrophage lineage. MDC has the four-cysteine motif and other highly conserved residues ... More

Modulation of cardiac Na+ channels expressed in a mammalian cell line and in ventricular myocytes by protein kinase C.

AuthorsQu Y, Rogers J, Tanada T, Scheuer T, Catterall WA

JournalProc Natl Acad Sci U S A

PubMed ID8159741

Cardiac rH1 Na+ channel alpha subunits were expressed in cells of the Chinese hamster lung 1610 cell line by transfection, and a stable cell line expressing cardiac Na+ channels (SNa-rH1) was isolated. Mean Na+ currents of 2.2 +/- 1.0 nA were recorded, which corresponds to a cell surface density of ... More

The p85 regulatory subunit controls sequential activation of phosphoinositide 3-kinase by Tyr kinases and Ras.

AuthorsJimenez C, Hernandez C, Pimentel B, Carrera AC,

JournalJ Biol Chem

PubMed ID12196526

Class IA phosphoinositide 3-kinase (PI3K) is a heterodimer composed of a p85 regulatory and a p110 catalytic subunit that regulates a variety of cell responses, including cell division and survival. PI3K is activated following Tyr kinase stimulation and by Ras. We found that the C-terminal region of p85, including the ... More

Suppression of growth of renal carcinoma cells by the von Hippel-Lindau tumor suppressor gene.

AuthorsChen F, Kishida T, Duh FM, Renbaum P, Orcutt ML, Schmidt L, Zbar B

JournalCancer Res

PubMed ID7585510

Clear cell renal carcinomas are most frequently characterized by loss of function of both copies of the von Hippel-Lindau (VHL) disease gene, suggesting that the VHL gene product plays an important role in regulating renal cell proliferation. To directly assess the function of the VHL gene product, we transfected the ... More

The zinc-finger protein slug causes desmosome dissociation, an initial and necessary step for growth factor-induced epithelial-mesenchymal transition.

AuthorsSavagner P, Yamada KM, Thiery JP

JournalJ Cell Biol

PubMed ID9182671

Epithelial-mesenchymal transition (EMT) is an essential morphogenetic process during embryonic development. It can be induced in vitro by hepatocyte growth factor/scatter factor (HGF/SF), or by FGF-1 in our NBT-II cell model for EMT. We tested for a central role in EMT of a zinc-finger protein called Slug. Slug mRNA and ... More

The UL12.5 gene product of herpes simplex virus type 1 exhibits nuclease and strand exchange activities but does not localize to the nucleus.

AuthorsReuven NB, Antoku S, Weller SK,

JournalJ Virol

PubMed ID15078942

The herpes simplex virus type 1 (HSV-1) alkaline nuclease, encoded by the UL12 gene, plays an important role in HSV-1 replication, as a null mutant of UL12 displays a severe growth defect. Although the precise in vivo role of UL12 has not yet been determined, several in vitro activities have ... More

Bax and Bak promote apoptosis by modulating endoplasmic reticular and mitochondrial Ca2+ stores.

Authors Nutt Leta K; Pataer Abujiang; Pahler Jessica; Fang Bingliang; Roth Jack; McConkey David J; Swisher Stephen G;

JournalJ Biol Chem

PubMed ID11741880

Alterations in intracellular Ca(2+) homeostasis and cytochrome c release from mitochondria have been implicated in the regulation of apoptosis, but the relationship between these events remains unclear. Here we report that enforced expression of either Bax or Bak via adenoviral gene delivery results in the accumulation of the proteins in ... More

Virosomes: cationic liposomes enhance retroviral transduction.

AuthorsHodgson CP, Solaiman F

JournalNat Biotechnol

PubMed ID9630897

Retrovirus-derived vectors are overwhelmingly preferred over other methods for ex vivo gene therapy because they provide permanent integration of foreign genes into cellular DNA. In comparison, cationic lipids mediate efficent gene transfer, but expression is transient. When we combined cationic lipids with retrovirus particles we obtained a significant enhancement of ... More

Efficient intracellular assembly of papillomaviral vectors.

AuthorsBuck CB, Pastrana DV, Lowy DR, Schiller JT,

JournalJ Virol

PubMed ID14694107

Although the papillomavirus structural proteins, L1 and L2, can spontaneously coassemble to form virus-like particles, currently available methods for production of L1/L2 particles capable of transducing reporter plasmids into mammalian cells are technically demanding and relatively low-yield. In this report, we describe a simple 293 cell transfection method for efficient ... More

The novel protein-tyrosine phosphatase PTP20 is a positive regulator of PC12 cell neuronal differentiation.

Authors Aoki N; Yamaguchi-Aoki Y; Ullrich A;

JournalJ Biol Chem

PubMed ID8910608

A novel cytoplasmic protein-tyrosine phosphatase (PTPase) designated PTP20 was isolated from a PC12 cDNA library and shown to positively regulate the differentiation process in PC12 cells. The PTP20 open reading frame of 453 amino acids contains a single tyrosine phosphatase catalytic domain and displays closest homology to members of the ... More

The mechanism of growth-inhibitory effect of DOC-2/DAB2 in prostate cancer. Characterization of a novel GTPase-activating protein associated with N-terminal domain of DOC-2/DAB2.

Authors Wang Zhi; Tseng Ching-Ping; Pong Rey-Chen; Chen Hong; McConnell John D; Navone Nora; Hsieh Jer-Tsong;

JournalJ Biol Chem

PubMed ID11812785

DOC-2/DAB2 is a member of the disable gene family with tumor-inhibitory activity. Its down-regulation is associated with several neoplasms, and serine phosphorylation of its N terminus modulates DOC-2/DAB2's inhibitory effect on AP-1 transcriptional activity. We describe the cloning of DIP1/2, a novel gene that interacts with the N-terminal domain of ... More

G beta gamma subunits mediate mitogen-activated protein kinase activation by the tyrosine kinase insulin-like growth factor 1 receptor.

Authors Luttrell L M; van Biesen T; Hawes B E; Koch W J; Touhara K; Lefkowitz R J;

JournalJ Biol Chem

PubMed ID7622449

The receptors for insulin-like growth factor 1 (IGF1) and insulin are related heterotetrameric proteins which, like the epidermal growth factor (EGF) receptor, possess intrinsic ligand-stimulated tyrosine protein kinase activity. In Rat 1 fibroblasts, stimulation of mitogen-activated protein (MAP) kinase via the IGF1 receptor and the Gi-coupled receptor for lysophosphatidic acid ... More

Underediting of glutamate receptor GluR-B mRNA in malignant gliomas.

Authors Maas S; Patt S; Schrey M; Rich A;

JournalProc Natl Acad Sci U S A

PubMed ID11717408

In mammals, RNA editing by site-selective adenosine deamination regulates key functional properties of neurotransmitter receptors in the central nervous system. Glutamate receptor subunit B is nearly 100% edited at one position (the Q/R-site), which is essential for normal receptor function. Its significance is apparent from mouse models in which a ... More

Requirement of b-myb function for survival and differentiative potential of human neuroblastoma cells.

Authors RaschellÃ G; Negroni A; Sala A; Pucci S; Romeo A; Calabretta B;

JournalJ Biol Chem

PubMed ID7721753

The B-myb gene belongs to a family of transcription factors that also includes A-myb and c-myb. B-myb is expressed in many cell types including human neuroblastoma cells. Here we demonstrate that B-myb expression is down-regulated during retinoic acid-induced neural and glial differentiation of neuroblastoma cells. This modulation is an early ... More

Estrogen lowers Alzheimer beta-amyloid generation by stimulating trans-Golgi network vesicle biogenesis.

Authors Greenfield Jeffrey P; Leung Lawrence W; Cai Dongming; Kaasik Krista; Gross Rachel S; Rodriguez-Boulan Enrique; Greengard Paul; Xu Huaxi;

JournalJ Biol Chem

PubMed ID11823458

Estrogen reduces the risk of Alzheimer's disease in post-menopausal women, beta-amyloid (Abeta) burden in animal models of Alzheimer's disease, and secretion of Abeta from neuronal cultures. The biological basis for these effects remains unknown. Here, utilizing cell-free systems derived from both neuroblastoma cells and primary neurons, we demonstrate that 17beta-estradiol ... More

CD44 anchors the assembly of matrilysin/MMP-7 with heparin-binding epidermal growth factor precursor and ErbB4 and regulates female reproductive organ remodeling.

Authors Yu Wei-Hsuan; Woessner J Frederick Jr; McNeish John D; Stamenkovic Ivan;

JournalGenes Dev

PubMed ID11825873

CD44 is a facultative proteoglycan implicated in cell adhesion and trafficking, as well as in tumor survival and progression. We demonstrate here that CD44 heparan sulfate proteoglycan (CD44HSPG) recruits proteolytically active matrix metalloproteinase 7 (matrilysin, MMP-7) and heparin-binding epidermal growth factor precursor (pro-HB-EGF) to form a complex on the surface ... More

The interferon (IFN)-induced GTPase, mGBP-2. Role in IFN-gamma-induced murine fibroblast proliferation.

Authors Gorbacheva Victoria Y; Lindner Daniel; Sen Ganes C; Vestal Deborah J;

JournalJ Biol Chem

PubMed ID11726669

To investigate the function of mGBP-2, a member of the interferon (IFN)-induced guanylate-binding protein family of GTPases, NIH 3T3 fibroblasts were generated that constitutively expressed mGBP-2. mGBP-2 induced a faster growth rate, with the highest expressing clones showing approximately a 50% reduction in doubling time. mGBP-2-expressing cells also grew to ... More

Functional analysis of the human D2 dopamine receptor missense variants.

Authors Cravchik A; Sibley D R; Gejman P V;

JournalJ Biol Chem

PubMed ID8824240

The human dopamine D2 receptor gene (DRD2) has three polymorphic variants that predict the amino acid substitutions Val96 --> Ala, Pro310 --> Ser, and Ser311 --> Cys in the receptor protein. We have investigated the ligand binding and signal transduction properties of these human D2 receptor variants by stably expressing ... More

Requirement of tyrosine residues 333 and 338 of the growth hormone (GH) receptor for selected GH-stimulated function.

Authors Lobie P E; Allevato G; Nielsen J H; Norstedt G; Billestrup N;

JournalJ Biol Chem

PubMed ID7665593

We have examined the involvement of tyrosine residues 333 and 338 of the growth hormone (GH) receptor in the cellular response to GH. Stable Chinese hamster ovary (CHO) cell clones expressing a receptor with tyrosine residues at position 333 and 338 of the receptor substituted for phenylalanine (CHO-GHR1-638 Y333F, Y338F) ... More

Ras mediates the activation of phospholipase D by v-Src.

Authors Jiang H; Lu Z; Luo J Q; Wolfman A; Foster D A;

JournalJ Biol Chem

PubMed ID7890731

We demonstrated previously that v-Src activates a phospholipase D (PLD) activity (Song, J., Pfeffer, L.M., and Foster, D.A. (1991) Mol. Cell. Biol. 11, 4903-4908) and that this activation is dependent upon a G protein(s) (Jiang H., Alexandropoulos, K., Song, J., and Foster, D.A. (1994) Mol. Cell. Biol. 14, 3676-3682). An ... More

The role of Tyr13 and Lys15 of interleukin-8 in the high affinity interaction with the interleukin-8 receptor type A.

Authors Schraufstätter I U; Ma M; Oades Z G; Barritt D S; Cochrane C G;

JournalJ Biol Chem

PubMed ID7737976

Interleukin-8 (IL-8) has at least two binding regions for both the A and the B type IL-8 receptors. This study defines an important region between Cys7 and Cys50 that, together with the Glu4-Leu5-Arg6 sequence of the NH2 terminus, accounts for the high affinity binding of IL-8 to the IL-8 A ... More

The human herpes virus 8-encoded viral FLICE inhibitory protein physically associates with and persistently activates the Ikappa B kinase complex.

Authors Liu Li; Eby Michael T; Rathore Nisha; Sinha Suwan K; Kumar Arvind; Chaudhary Preet M;

JournalJ Biol Chem

PubMed ID11830587

The human herpesvirus 8 (HHV8, also called Kaposi's sarcoma-associated herpesvirus) has been linked to Kaposi's sarcoma and primary effusion lymphoma (PEL) in immunocompromised individuals. We demonstrate that PEL cell lines have a constitutively active NF-kappaB pathway, which is associated with persistent phosphorylation of IkappaBalpha. To elucidate the mechanism of NF-kappaB ... More

TRAIL-induced apoptosis requires Bax-dependent mitochondrial release of Smac/DIABLO.

Authors Deng Yibin; Lin Yahong; Wu Xiangwei;

JournalGenes Dev

PubMed ID11782443

Recent reports suggest that a cross-talk exists between apoptosis pathways mediated by mitochondria and cell death receptors. In the present study, we report that mitochondrial events are required for apoptosis induced by the cell death ligand TRAIL (TNF-related apoptosis-inducing ligand) in human cancer cells. We show that the Bax null ... More

HER4-mediated biological and biochemical properties in NIH 3T3 cells. Evidence for HER1-HER4 heterodimers.

Authors Cohen B D; Green J M; Foy L; Fell H P;

JournalJ Biol Chem

PubMed ID8617750

The EGF receptor family of tyrosine kinase growth factor receptors is expressed in a variety of cell types and has been implicated in the progression of certain human adenocarcinomas. The most recent addition to this family of receptors, HER4, was expressed in NIH 3T3 cells to determine its biological and ... More

Identification in Drosophila melanogaster of the invertebrate G protein-coupled FMRFamide receptor.

Authors Meeusen Tom; Mertens Inge; Clynen Elke; Baggerman Geert; Nichols Ruthann; Nachman Ronald J; Huybrechts Roger; De Loof Arnold; Schoofs Liliane;

JournalProc Natl Acad Sci U S A

PubMed ID12438685

We here describe the cloning and characterization of the functionally active Drosophila melanogaster (Drm) FMRFamide receptor, which we designated as DrmFMRFa-R. The full-length ORF of a D. melanogaster orphan receptor, CG 2114 (Berkeley Drosophila Genome Project), was cloned from genomic DNA. This receptor is distantly related to mammalian thyroid-stimulating hormone-releasing ... More

US2, a human cytomegalovirus-encoded type I membrane protein, contains a non-cleavable amino-terminal signal peptide.

Authors Gewurz Benjamin E; Ploegh Hidde L; Tortorella Domenico;

JournalJ Biol Chem

PubMed ID11790769

The human cytomegalovirus US2 gene product targets major histocompatibility class I molecules for degradation in a proteasome-dependent fashion. Degradation requires interaction between the endoplasmic reticulum (ER) lumenal domains of US2 and class I. While ER insertion of US2 is essential for US2 function, US2 lacks a cleavable signal peptide. Radiosequence ... More

p22phox is a critical component of the superoxide-generating NADH/NADPH oxidase system and regulates angiotensin II-induced hypertrophy in vascular smooth muscle cells.

Authors Ushio-Fukai M; Zafari A M; Fukui T; Ishizaka N; Griendling K K;

JournalJ Biol Chem

PubMed ID8798532

Superoxide anion formation is vital to the microbicidal activity of phagocytes. Recently, however, there is accumulating evidence that it is also involved in cell growth in vascular smooth muscle cells (VSMCs). We have shown that the hypertrophic agent angiotensin II stimulates superoxide production by activating the membrane-bound NADH/NADPH oxidase and ... More

Bax-mediated Ca2+ mobilization promotes cytochrome c release during apoptosis.

Authors Nutt Leta K; Chandra Joya; Pataer Abujiang; Fang Bingliang; Roth Jack A; Swisher Stephen G; O'Neil Roger G; McConkey David J;

JournalJ Biol Chem

PubMed ID11909872

Previous studies have demonstrated that Ca(2+) is released from the endoplasmic reticulum (ER) in some models of apoptosis, but the mechanisms involved and the functional significance remain obscure. We confirmed that apoptosis induced by some (but not all) proapoptotic stimuli was associated with caspase-independent, BCL-2-sensitive emptying of the ER Ca(2+) ... More

The forkhead transcription factor AFX activates apoptosis by induction of the BCL-6 transcriptional repressor.

Authors Tang Tracy Tzu-Ling; Dowbenko Donald; Jackson Amanda; Toney Lisa; Lewin David A; Dent Alexander L; Lasky Laurence A;

JournalJ Biol Chem

PubMed ID11777915

The activation of the AKT/protein kinase B kinases by mutation of the PTEN lipid phosphatase results in enhanced survival of a diversity of tumors. This resistance to apoptosis is partly accomplished by the inhibition of genetic programs induced by a subfamily of forkhead transcription factors including AFX. Here we describe ... More

The first proline of PALP motif at the C terminus of presenilins is obligatory for stabilization, complex formation, and gamma-secretase activities of presenilins.

Authors Tomita T; Watabiki T; Takikawa R; Morohashi Y; Takasugi N; Kopan R; De Strooper B; Iwatsubo T;

JournalJ Biol Chem

PubMed ID11432849

Mutations in presenilin (PS) genes cause early-onset familial Alzheimer's disease by increasing production of the amyloidogenic form of amyloid beta peptides ending at residue 42 (Abeta42). PS is an evolutionarily conserved multipass transmembrane protein, and all known PS proteins contain a proline-alanine-leucine-proline (PALP) motif starting at proline (P) 414 (amino ... More

Overexpression of ganglioside GM1 results in the dispersion of platelet-derived growth factor receptor from glycolipid-enriched microdomains and in the suppression of cell growth signals.

Authors Mitsuda Teruhiko; Furukawa Keiko; Fukumoto Satoshi; Miyazaki Hiroshi; Urano Takeshi; Furukawa Koichi;

JournalJ Biol Chem

PubMed ID11782461

To investigate the molecular mechanisms of gangliosides for the regulation of cell proliferation, Swiss 3T3 cells were transfected with GM2/GD2 synthase and GM1 synthase cDNAs, resulting in the establishment of GM1-expressing (GM1(+)) lines. Compared with the vector control (GM1(-)) cell lines, GM1(+) cells exhibited reduced cell proliferation by stimulation with ... More

Differential signaling of cyclic AMP: opposing effects of exchange protein directly activated by cyclic AMP and cAMP-dependent protein kinase on protein kinase B activation.

Authors Mei Fang C; Qiao Jingbo; Tsygankova Oxana M; Meinkoth Judy L; Quilliam Lawrence A; Cheng Xiaodong;

JournalJ Biol Chem

PubMed ID11801596

The recent discovery of Epac, a novel cAMP receptor protein, opens up a new dimension in studying cAMP-mediated cell signaling. It is conceivable that many of the cAMP functions previously attributed to cAMP-dependent protein kinase (PKA) are in fact also Epac-dependent. The finding of an additional intracellular cAMP receptor provides ... More

Wortmannin-sensitive activation of p70s6k by endogenous and heterologously expressed Gi-coupled receptors.

Authors Wilson M; Burt A R; Milligan G; Anderson N G;

JournalJ Biol Chem

PubMed ID8621477

In order to study the regulation of the ribosomal protein S6 kinase, p70s6k, by G protein-coupled receptors, Rat-1 fibroblasts were stably transfected with two versions of the alpha2 adrenergic receptor. Stimulation of clone 1C cells, which express 3.5 pmol/mg of protein of the human alpha2C10 receptor, with the alpha2 agonist ... More

CD45 controls interleukin-4-mediated IgE class switch recombination in human B cells through its function as a Janus kinase phosphatase.

Authors Yamada Takechiyo; Zhu Daocheng; Saxon Andrew; Zhang Ke;

JournalJ Biol Chem

PubMed ID11994288

CD45 plays a critical regulatory role in receptor signaling through its protein tyrosine phosphatase and Janus kinase (JAK) phosphatase activities. To investigate whether CD45 also plays a regulatory role in Ig class switching in human B cells, we examined the effects of CD45 triggering on Ig class switching to IgE ... More

A somatic cell genetic system for dissecting hemopoietic cytokine signal transduction.

Authors Richardson Rachael T; Starr Robyn; Angus Leecia J L; Hilton Douglas J;

JournalJ Biol Chem

PubMed ID11994291

Somatic cell genetics has proven to be a powerful tool for the dissection of cytokine signal transduction pathways. Here we describe a system in which interleukin-6 (IL-6) signaling may be dissected using myeloid leukemic M1 cells. We utilized two properties of M1 cell differentiation to isolate IL-6-unresponsive mutants. First, M1 ... More