E. coli Expression System with Gateway® Technology

Catalog number: 11824026

Invitrogen™  Related applications: Protein Expression

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The E. coli Expression System with Gateway™ Technology is designed to create E. coli expression clones containing the T7 promoter. The expression clones are ready for transformation and expression in E. coli. The system offers:
For Research Use Only. Not for use in diagnostic procedures.


Expression: E. coli
Promoter: T7
Bacterial or Yeast Strain: BL21-AI™
Antibiotic Resistance (Bacterial): Ampicillin (AmpR)
Cloning Method: Gateway®
Expression Mechanism: Cell-Based Expression
Product Line: Gateway®
Product Size: 20 reactions
Protein Tag or Fusion: GST Tag, His Tag (6x)
Vector Type: Gateway T7 Vectors

Contents & storage

The E. coli Expression System with Gateway™ Technology includes: LR Clonase® enzyme mix and reaction buffer, Gateway™ destination vectors pDEST™14, pDEST™15, pDEST17, and pDEST™24, proteinase K solution (2 µg/µl), pENTR™-gus positive control, One Shot® BL21-AI™ and Library Efficiency® DH5α™ Competent Cells. Store component cells and Clonase® enzyme mixes at -70°C. Store vectors at -20°C.


Manuals & protocols