The E. coli Expression System with Gateway™ Technology is designed to create E. coli expression clones containing the T7 promoter. The expression clones are ready for transformation and expression in E. coli. The system offers:
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Promoter
T7
Product Type
Expression System
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Bacterial or Yeast Strain
BL21-AI™
Expression System
E. coli
Expression Mechanism
Cell-Based Expression
Protein Tag
GST Tag, His Tag (6x)
Cloning Method
Gateway
Vector
Gateway T7 Vectors
Product Line
Gateway™
Contents & Storage
The E. coli Expression System with Gateway™ Technology includes: LR Clonase™ enzyme mix and reaction buffer, Gateway™ destination vectors pDEST™14, pDEST™15, pDEST™ 17, and pDEST™24, proteinase K solution (2 µg/µl), pENTR™-gus positive control, One Shot™ BL21-AI™ and Library Efficiency™ DH5α™ Competent Cells. Store component cells and Clonase™ enzyme mixes at -70°C. Store vectors at -20°C.