Baculovirus Expression System with Gateway® Technology

Catalog number: 11827011

Invitrogen™  Related applications: Protein Expression

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Description

The Baculovirus Expression System with Gateway® Technology is designed to create recombinant pFastBac™ plasmids containing the polyhedrin promoter. The Baculovirus Expression System with Gateway® Technology offers:

• Expression using the Bac-to-Bac® technology (1)
• Destination vectors carrying the polyhedrin promoter for production of native (pDEST™8), N-terminal histidine fusion (pDEST™10), and N-terminal GST fusion (pDEST™20) proteins
• pDEST™10 vector containing a TEV protease cleavage site for removal of the fusion tag after protein purification (2)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: Polyhedrin
Vector Type: Gateway Baculovirus Vectors, pDEST
Product Size: 20 reactions
Cloning Method: Gateway®
Protein Tag or Fusion: His Tag (6x), Untagged, GST Tag

Contents & storage

The Baculovirus Expression System with Gateway® Technology includes LR Clonase® enzyme mix and reaction buffer, destination vectors pDEST™8, pDEST™10, and pDEST™20, proteinase K solution (2 µg/µl), pENTR™-gus positive control, and Library Efficiency® DH5Competent Cells. The kit does not include MAX Efficiency® DH10Bac™ Competent Cells. Store LR Clonase® enzyme mix and Competent Cells at -80°C. Store all other components at -20°C. Components are guaranteed stable for 6 months when properly stored.

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