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Custom Primer - FAQs

View additional product information for Custom Primer - FAQs (11846037)

4 product FAQs found

What is the smallest quantity of RNA detectable by the SuperScript IV First-Strand System for RT-PCR?

Detection limits dependend on many factors, including primer design, target size, and the abundance of mRNA. The recommended template amount when working with SuperScript IV Reverse Transcriptase is 10 pg-5 µg total RNA or 10 pg-500 ng mRNA.

What size targets can be amplified by the SuperScript One-Step RT-PCR Systems?

The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is recommended for amplifying RNA targets up to 4.5 kb and the SuperScript IV UniPrime One-Step RT-PCR System is recommended for amplifying RNA targets up to 13 kb.

How stable are your cationic lipids that are used in transfection?

All the following lipids are stable at 4º C for at least one year:
11668-019 Lipofectamine 2000
10362-100 Cellfectin II
10459-014 DMRIE-C
10964-013 Lipofectamine PLUS
18292-011 Lipofectin
18324-012 Lipofectamine


Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I sequence a peptide that is acetylated or biotinylated?

No. These groups effectively block the N terminus.