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IL4 Recombinant Human Protein

Catalog number: 11846HNAE5

 Related applications: Protein Assays and Analysis

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Description

Interleukin 4 (IL-4), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications

This recombinant protein was expressed from a DNA sequence encoding the mature form of human IL4 isoform 1 (P05112-1) (His 25-Ser 153) retaining the initial Met.

Activity: Measured in a cell proliferation assay using TF1 human erythroleukemic cells (Kitamura, T. et al.,1989, J. Cell Physiol. 140:323.) . The EC50 for this effect is typically 0.1-0.3 ng/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Research Category: Immunology, Signal Transduction
Expression: E. coli
Form: Lyophilized
Gene Aliases: BCGF-1, BCGF1, BSF1, IL-4, MGC79402
Gene Symbol: IL4
Molecular Weight: 15 kDa
Product Line: Sino Biological®
Product Size: 5 µg
Protein Family: Cytokines & Receptors
Protein Form: Full Length, Recombinant
Protein Name: interleukin 4
Protein Subtype: Interleukins
Protein Tag or Fusion: Untagged
Purity: ≥ 90% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols

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