RPMI 1640 Medium
RPMI 1640 Medium
RPMI 1640 Medium
RPMI 1640 Medium
Gibco™

RPMI 1640 Medium

RPMI 1640 Medium wurde ursprünglich zur Züchtung leukämischer Humanzellen in Suspension und als Monolayer entwickelt. Roswell Park Memorial Institute (RPMI)Weitere Informationen
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KatalognummerMenge
11875093500 mL
11875101100 mL
118750851.000 mL
Katalognummer 11875093
Preis (EUR)
32,65
Exklusiv online
35,53
Ersparnis 2,88 (8%)
Each
Zum Warenkorb hinzufügen
Menge:
500 mL
Customize this product
Preis (EUR)
32,65
Exklusiv online
35,53
Ersparnis 2,88 (8%)
Each
Zum Warenkorb hinzufügen
RPMI 1640 Medium wurde ursprünglich zur Züchtung leukämischer Humanzellen in Suspension und als Monolayer entwickelt. Roswell Park Memorial Institute (RPMI) 1640 Medium wurde seitdem für eine Reihe von Säugetierzellen wie HeLa, Jurkat, MCF-7, PC12, PBMC, Astrozyten und Karzinome als geeignet befunden. Wir bieten verschiedene RPMI 1640 Medium-Modifikationen für eine Vielzahl von Zellkulturanwendungen an. Finden Sie die richtige Zusammensetzung mithilfe des Medien-Auswahlwerkzeuges.

Dieses RPMI wurde wie folgt modifiziert:
Mit  Ohne
• L-Glutamin  • HEPES
• Phenolrot 

Die vollständige Zusammensetzung ist verfügbar.

Verwendung von RPMI
RPMI 1640 Medium unterscheidet sich deutlich von anderen Medien, da es das Reduktionsmittel Glutathion sowie hohe Konzentrationen an Vitaminen enthält. RPMI 1640 Medium enthält Biotin, Vitamin B12 und PABA, welche nicht in Eagle's Minimal Essential Medium bzw. Dulbecco's Modified Eagle Medium enthalten sind. Darüber hinaus sind die Vitamine Inositol und Cholin in sehr hohen Konzentrationen enthalten. RPMI 1640 Medium enthält keine Proteine, Lipide oder Wachstumsfaktoren. Aus diesem Grund erfordert RPMI 1640 Medium eine Supplementierung, gewöhnlich mit 10 % fetalem Rinderserum (FBS). RPMI 1640 Medium verwendet ein Natriumbikarbonat-Puffersystem (2,0 g/l) und erfordert daher eine CO–2-Umgebung von 5 bis 10 %, um den physiologischen pH-Wert aufrechtzuerhalten.

cGMP-konformes Herstellungs- und Qualitätssystem
RPMI 1640 Medium wird in einer cGMP-konformen Einrichtung in Grand Island, New York, hergestellt. Die Einrichtung ist von der US-Arzneimittelbehörde FDA als Hersteller von Medizinprodukten zugelassen und nach ISO 13485-zertifiziert. Zur Gewährleistung einer ununterbrochenen Versorgungskette bieten wir ein identisches RPMI 1640-Produkt aus unserem Betrieb in Schottland an (21875-158). Diese Einrichtung ist von der US-Arzneimittelbehörde FDA als Hersteller von Medizinprodukten zugelassen und ISO 13485-zertifiziert.
Für die Verwendung in der Forschung oder die Weiterverarbeitung. Nicht zur Diagnosestellung oder direkten Anwendung bei Menschen oder Tieren geeignet.
Specifications
ZelllinieHeLa, Jurkat, MCF-7, PC-12, PBMC, Astrozyten und Karzinome
ZelltypLeukämische Zellen
Konzentration1 X
Fertigungsqualität, HerstellungsqualitätcGMP-compliant under the ISO 13485 standard
ProduktlinieGibco
ProdukttypRPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Menge500 mL
Haltbarkeit12 Monate ab Herstellungsdatum
VersandbedingungRaumtemperatur
KlassifikationOhne Stoffe tierischen Ursprungs
FormFlüssig
Serum LevelStandard-Serumzugabe
SterilitätSteril gefiltert
Sterilization MethodSterile-filtered
Mit AdditivenGlutamin, Phenolrot
Ohne AdditiveKeine HEPES, Ohne Natriumpyruvat
Unit SizeEach
Inhalt und Lagerung
Lagerbedingungen: 2 °C bis 8 °C (vor Licht schützen)
Versandbedingungen: Haltbarkeit bei
Raumtemperatur: 12 Monate ab Herstellungsdatum

Häufig gestellte Fragen (FAQ)

How light sensitive is RPMI 1640 media? Should I also be protecting it from LED light?

While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the density (g/L) for RPMI 1640 Medium?

We have specific gravity information for RPMI 1640 Medium: 1.006 kg/L. In this case, the specific gravity is the same as density as the solvent is water.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Zitierungen und Referenzen (16)

Zitierungen und Referenzen
Abstract
Smac agonists sensitize for Apo2L/TRAIL- or anticancer drug-induced apoptosis and induce regression of malignant glioma in vivo.
Authors: Fulda Simone; Wick Wolfgang; Weller Michael; Debatin Klaus-Michael;
Journal:Nat Med
PubMed ID:12118245
'A major concern in cancer therapy is resistance of tumors such as glioblastoma to current treatment protocols. Here, we report that transfer of the gene encoding second mitochondria-derived activator of caspase (Smac) or Smac peptides sensitized various tumor cells in vitro and malignant glioma cells in vivo for apoptosis induced ... More
Simulated microgravity culture system for a 3-D carcinoma tissue model.
Authors:Nakamura K, Kuga H, Morisaki T, Baba E, Sato N, Mizumoto K, Sueishi K, Tanaka M, Katano M,
Journal:Biotechniques
PubMed ID:12449385
'An in vitro organotypic culture model is needed to understand the complexities of carcinoma tissue consisting of carcinoma cells, stromal cells, and extracellular matrices. We developed a new in vitro model of carcinoma tissue using a rotary cell culture system with four disposable vessels (RCCS-4D) that provides a simulated microgravity ... More
The RasGAP N-terminal fragment generated by caspase cleavage protects cells in a Ras/PI3K/Akt-dependent manner that does not rely on NFkappa B activation.
Authors: Yang Jiang-Yan; Widmann Christian;
Journal:J Biol Chem
PubMed ID:11847220
'RasGAP, a regulator of Ras GTPase family members, is cleaved at low levels of caspase activity into an N-terminal fragment (fragment N) that generates potent anti-apoptotic signals. At higher levels of caspase activity, fragment N is further cleaved into two fragments that strongly potentiate apoptosis. RasGAP could thus function as ... More
The p53-activated gene, PAG608, requires a zinc finger domain for nuclear localization and oxidative stress-induced apoptosis.
Authors: Higashi Youichirou; Asanuma Masato; Miyazaki Ikuko; Haque M Emdadul; Fujita Naoko; Tanaka Ken-Ichi; Ogawa Norio;
Journal:J Biol Chem
PubMed ID:12196512
'The p53-activated gene PAG608, which encodes a nuclear zinc finger protein, is a p53-inducible gene that contributes to p53-mediated apoptosis. However, the mechanisms by which PAG608 is involved in the apoptosis of neuronal cells are still obscure. In this study, we demonstrated that expression of p53 was induced by 100 ... More
Size, concentration and incubation time dependence of gold nanoparticle uptake into pancreas cancer cells and its future application to X-Ray Drug Delivery System.
Authors:Trono JD, Mizuno K, Yusa N, Matsukawa T, Yokoyama K, Uesaka M
Journal:J Radiat Res
PubMed ID:21187668
'One of the restrictions in the potential use of gold markers for medical imaging/tracking of harder tumors is its size. We propose to use gold nanoparticles which, due to its small size, can be administered conveniently via intravenous injection. One of the factors that determine the clinical utility of nanoparticles ... More