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IFNL3 (IL28B) Recombinant Human Protein, His Tag

Catalog number: 11890H08H50

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Description

Interferon Lambda 3 (IFNL3), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications

This recombinant protein was expressed from a DNA sequence encoding the human IFNL3 (NP_742151.2) (Met 1-Val 196) fused to a polyhistidine tag at the C-terminus.

Activity:
1. Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV). The EC50 for this effect is typically 4-20 ng/ml.

2. Measured by its binding ability in a functional ELISA. Immobilized human IL28B at 20 µg/ml (100 µl/well) can bind human IL10RB with a linear ranger of 3.125-25 µg/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 500 mM NaCl, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Research Category: Immunology
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: IFNL3, IL-28B, IL28C
Gene Symbol: IFNL3
Molecular Weight: 21 kDa
Product Line: Sino Biological®
Product Size: 50 µg
Protein Family: Cytokines & Receptors
Protein Name: interferon lambda 3
Protein Subtype: Interferons, Interleukins
Protein Tag or Fusion: His Tag
Purity: ≥ 90% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols

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