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Gibco™

MEM α, nucleosides, powder

Catalog number:  11900016

 Related applications:

Mammalian Cell Culture

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Description

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco® MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM α is modified as follows:

With Without
• Ribonucleosides • Sodium Bicarbonate
• Deoxyribonucleosides
• L-glutamine
• Phenol Red

The complete formulation is available.

Gibco® MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle’s salts.

cGMP Manufacturing and Quality System
Gibco® MEM α is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco® MEM α product made in our Grand Island facility (11900-024). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red
Form: Powder
HEPES Buffer: No HEPES
Sodium Pyruvate Additive: Sodium Pyruvate
Classification: Animal Origin-Free
Culture Environment: CO2
Osmolality: 240 - 290 mOsm/kg
Product Line: Gibco®
Product Size: 1 L
Serum Supplementation: Standard Serum Supplementation
Sodium Bicarbonate Buffer: No Sodium Bicarbonate
Supplementation Required: Serum, Sodium Bicarbonate
pH Range: 3.3 - 3.9
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8° C.
Shipping conditions: Ambient.
Shelf life: 36 months from date of manufacture.

Documents

Manuals & protocols