MEM α, nucleosides, powder

Catalog number: 11900016

Gibco™  Related applications: Mammalian Cell Culture

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Description

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco® MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM α is modified as follows:

With Without
• Ribonucleosides • Sodium Bicarbonate
• Deoxyribonucleosides
• L-glutamine
• Phenol Red

The complete formulation is available.

Gibco® MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle’s salts.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® MEM α is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco® MEM α product made in our Grand Island facility (11900-024). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Specifications

Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red
Form: Powder
HEPES Buffer: No HEPES
Sodium Pyruvate Additive: Sodium Pyruvate
Classification: Animal Origin-Free
Culture Environment: CO2
Osmolality: 240 - 290 mOsm/kg
Product Size: 1 L
Serum Supplementation: Standard Serum Supplementation
Sodium Bicarbonate Buffer: No Sodium Bicarbonate
Supplementation Required: Serum, Sodium Bicarbonate
pH Range: 3.3 - 3.9
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8° C.
Shipping conditions: Ambient.
Shelf life: 36 months from date of manufacture.

Documents

Manuals & protocols

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