Invitrogen™

SuperScript™ Double-Stranded cDNA Synthesis Kit

Catalog number:  11917020

Related applications:

Nucleic Acid Labeling & Oligo Synthesis

| Reverse Transcription

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Description

The SuperScript® Double-Stranded cDNA Synthesis Kit contains all of the reagents (except an oligo (dT)-containing primer) necessary to make high-quality, double-stranded cDNA from total RNA or poly A+-selected RNA (mRNA). The advanced capabilities of SuperScript® II Reverse Transcriptase, with its reduced RNase H activity, maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis. Features of the SuperScript® Double-Stranded cDNA Synthesis Kit include:

Reliability—each buffer, reagent, and enzyme in the kit is of the same high quality you expect from us
Performance—SuperScript® II is an enzyme you can trust for high-yield, high-quality, general-purpose, first-strand synthesis.

Simple, reliable, and trouble-free
The SuperScript® Double-Stranded cDNA Synthesis Kit offers a simple, reliable, and trouble-free method by which RNA templates can be converted to cDNA for use in cloning and library construction experiments. The reactions described in the SuperScript® Double-Stranded cDNA Synthesis Kit protocol are designed to convert 25–50 µg total RNA, or 0.2–5 µg mRNA, into first- and second-strand cDNA (supply your own primer). Enjoy the benefits of a tested protocol and reagents of consistent quality. To get the best possible cDNA product using this kit, we recommend the use of a high-quality RNA substrate prepared using TRIzol® RNA Isolation Reagents.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Final Product Type: cDNA (Double-Stranded)
Labeling Method: Direct Labeling
Optimal Reaction Temperature: 42° C
Product Line: SuperScript™
Reaction Format: Separate Components
Reverse Transcriptase: SuperScript™ II
Sample Type: RNA
Shipping Condition: Wet Ice
For Use With (Application): Microarray Analysis
Quantity: 50 reactions

Contents & storage

The kit includes reagents and buffers for 10 first- and second-strand cDNA synthesis reactions converting 25-50 µg total RNA or 0.2-5 µg mRNA into double-stranded cDNA. All components are guaranteed stable for 6 months when properly stored.