Invitrogen™

SuperScript™ Double-Stranded cDNA Synthesis Kit

Catalog number: 11917020
Invitrogen™

SuperScript™ Double-Stranded cDNA Synthesis Kit

Catalog number: 11917020
Catalog Number
11917020
also known as 11917-020
Unit Size
50 rxns
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
10 rxns
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
11917020
also known as 11917-020
50 rxns
-
1191701010 rxns
-
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The SuperScript™ Double-Stranded cDNA Synthesis Kit contains all of the reagents (except an oligo (dT)-containing primer) necessary to make high-quality, double-stranded cDNA from total RNA or poly A+-selected RNA (mRNA). The advanced capabilities of SuperScript™ II Reverse Transcriptase, with its reduced RNase H activity, maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis. Features of the SuperScript™

Double-Stranded cDNA Synthesis Kit include:

Reliability—each buffer, reagent, and enzyme in the kit is of the same high quality you expect from us
Performance—SuperScript™ II is an enzyme you can trust for high-yield, high-quality, general-purpose, first-strand synthesis.

Simple, reliable, and trouble-free
The SuperScript™ Double-Stranded cDNA Synthesis Kit offers a simple, reliable, and trouble-free method by which RNA templates can be converted to cDNA for use in cloning and library construction experiments. The reactions described in the SuperScript™ Double-Stranded cDNA Synthesis Kit protocol are designed to convert 25–50 μg total RNA, or 0.2–5 μg mRNA, into first- and second-strand cDNA (supply your own primer). Enjoy the benefits of a tested protocol and reagents of consistent quality. To get the best possible cDNA product using this kit, we recommend the use of a high-quality RNA substrate prepared using TRIzol™ RNA Isolation Reagents.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Kit
Labeling Method
Direct Labeling
Optimal Reaction Temperature
42°C
Product Line
SuperScript™
Product Type
cDNA Synthesis Kit
Quantity
50 Reactions
Reverse Transcriptase
SuperScript™ II
Shipping Condition
Wet Ice
For Use With (Application)
Microarray Analysis
Final Product Type
cDNA (Double-Stranded)
Sample Type
RNA

Contents & Storage

The kit includes reagents and buffers for 10 first- and second-strand cDNA synthesis reactions converting 25-50 μg total RNA or 0.2-5 μg mRNA into double-stranded cDNA. All components are guaranteed stable for 6 months when properly stored.

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