For superior RT-PCR performance, upgrade to the new SuperScript IV One-Step RT-PCR System.

SuperScript™ One-Step RT-PCR System for Long Templates

Catalog number:  11922028

Invitrogen™  Related applications: Reverse Transcription

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The SuperScript One-Step RT-PCR System for Long Templates is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR. The system consists of two major components: SuperScript II RT/Platinum Taq Hi Fi Mix and 2X Reaction Mix. The enzyme mix is a mixture of SuperScript II Reverse Transcriptase and Platinum Taq DNA Polymerase High Fidelity, which is a blend of recombinant Taq DNA polymerase and the proofreading enzyme Pyrococcus species GB-D thermostable polymerase. The Taq DNA polymerase in the blend is complexed with Platinum antibodies that inhibit enzyme activity at ambient temperatures enabling hot start PCR. The 2X Reaction Mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg2+ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers. All components for cDNA synthesis and PCR are combined in a single tube with gene-specific primers and target RNA. Reverse transcription automatically follows PCR cycling without additional steps. This enzyme mixture can amplify RNA targets up to 9.0 kb.

Note: For superior one-step RT-PCR performance, we recommend the SuperScript IV One-Step RT-PCR System or SuperScript IV One-Step RT-PCR System with ezDNase. The SuperScript IV One-Step RT-PCR System combines the high processivity of SuperScript IV Reverse Transcriptase with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched product yields, specificity, and sensitivity in less time and for a broad target range, even with suboptimally pure RNA samples.

For Research Use Only. Not for use in diagnostic procedures.


PCR Method: 1-Step RT-PCR
Reverse Transcriptase: SuperScript™ II
Polymerase: Platinum® Taq DNA Polymerase High Fidelity
Hot Start: Built-In Hot Start
Sample Type (General): RNA
Detection Method: Gel Electrophoresis
Final Product Size(s): 9 kb or less
Primer-Probe Compatibility: Unlabeled Oligos
Product Line: Platinum®, SuperScript™
Product Size: 100 reactions
Shipping Condition: Wet Ice

Contents & storage

SuperScript® One-Step RT-PCR System components are listed in Table 1. Store at -20°C. Guaranteed stable for 6 months when properly stored.


Manuals & protocols

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