Gibco™

MEM (Temin's modification) (2X), no phenol red

Catalog number: 11935046
Gibco™

MEM (Temin's modification) (2X), no phenol red

Catalog number: 11935046
Catalog Number
11935046
Unit Size
500 mL
Price (USD)
Price: 65.50
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
11935046500 mL
Price: 65.50
Your Price:
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations
Temin's Modified Eagle Medium (MEM), is a modified form of Minimum Essential Medium, one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With Without
• L-glutamine • HEPES
• Earle's salts • Phenol Red
• Sodium Bicarbonate

The complete formulation is available.

MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. Temin’s Modification is a 2x MEM with 4x the vitamins and amino acids of the original MEM. This MEM formulation contains Earle’s salts for use in a CO2 incubator. This product is made with Earle’s salts.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing
Gibco™ MEM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (8.4 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Specifications

Cell Line
HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell Type
Primary Rat Astrocytes
Classification
Animal Origin-Free
Concentration
1 X
Form
Liquid
Product Type
MEM (Minimum Essential Medium)
Shelf Life
12 Months From Date of Manufacture
Sterility
Sterile-filtered
With Additives
High Glucose, Glutamine, Earle's Salts, Sodium Bicarbonate
Without Additives
No HEPES, No Phenol Red, No Sodium Pyruvate
Green Features
Sustainable packaging
Product Line
Gibco™
Quantity
500 mL
Shipping Condition
Room Temperature

Contents & Storage

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture