Invitrogen Platinum Taq
Green Hot Start DNA Polymerase provides Platinum Taq
DNA Polymerase with a 10X Green PCR Buffer. Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq
DNA Polymerase. The 'hot start' property of the enzyme is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step, thus preventing the extention of nonspecifically annealed primers and improving product yield.
Features of Platinum Taq
Green Hot Start DNA Polymerase include:
• High specificity and increased yields with antibody-mediated hot-start PCR
• Convenient room-temperature reaction setup
• Direct gel loading with green PCR buffer
• Versatile formulation for broad range of ampliconsAbout Platinum Taq DNA Polymerase
DNA Polymerase is a recombinant Taq
DNA polymerase complexed with proprietary Platinum antibodies. The antibodies dissociate during the initial PCR denaturation step and the DNA polymerase regains its full activity. Just as with Taq
DNA Polymerase, Platinum Taq
DNA Polymerase has a non-template-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5'→3' exonuclease activity.
Green Hot Start DNA Polymerase is provided with green PCR buffer that contains a density reagent and two tracking dyes and allows for direct gel loading of PCR products. The optional KB Extender used with Platinum Taq
DNA Polymerase enables more versatility in PCR assays with long or GC-rich amplicons. Use Platinum Taq Green Hot Start DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR. Note:
For superior PCR performance, we recommend the next-generation enzyme Platinum II Taq Hot-start DNA Polymerase
. Platinum II Taq
Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq
DNA polymerase, and superior hot-start technology. Platinum II Taq
Hot-Start DNA Polymerase can be used with Platinum II Green PCR Buffer that is supplemented with a density reagent and two tracking dyes for direct loading of PCR products on gels.
For Research Use Only. Not for use in diagnostic procedures.