For superior PCR performance, upgrade to Invitrogen's new Platinum II Taq Hot-start DNA Polymerase.
Invitrogen™

Platinum™ Taq Green Hot Start DNA Polymerase

Catalog number:  11966034

 Related applications: Genotyping & Genomic Profiling | Methylation Analysis | PCR | Sanger Sequencing

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Description

Invitrogen Platinum Taq Green Hot Start DNA Polymerase provides Platinum Taq DNA Polymerase with a 10X Green PCR Buffer. Platinum Taq DNA Polymerase is a convenient and reliable "hot start" thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. The "hot start" property of the enzyme is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step, thus preventing the extention of nonspecifically annealed primers and improving product yield.

Features of Platinum Taq Green Hot Start DNA Polymerase include:
• High specificity and increased yields with antibody-mediated hot-start PCR
• Convenient room-temperature reaction setup
• Direct gel loading with green PCR buffer
• Versatile formulation for broad range of amplicons

About Platinum Taq DNA Polymerase
Platinum Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with proprietary Platinum antibodies. The antibodies dissociate during the initial PCR denaturation step and the DNA polymerase regains its full activity. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5'→3' exonuclease activity.

Platinum Taq Green Hot Start DNA Polymerase is provided with green PCR buffer that contains a density reagent and two tracking dyes and allows for direct gel loading of PCR products. The optional KB Extender used with Platinum Taq DNA Polymerase enables more versatility in PCR assays with long or GC-rich amplicons. Use Platinum Taq Green Hot Start DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR.

Note: For superior PCR performance, we recommend the next-generation enzyme Platinum II Taq Hot-start DNA Polymerase. Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology. Platinum II Taq Hot-Start DNA Polymerase can be used with Platinum II Green PCR Buffer that is supplemented with a density reagent and two tracking dyes for direct loading of PCR products on gels.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: Platinum™ Taq DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Reaction Format: Separate Components
Product Overhang: 3'-A
Reaction Speed: Standard
Final Product Size(s): 5 kb or less
Product Line: Platinum™
Product Size: 600 reactions
Shipping Condition: Dry Ice

Contents & storage

Each box contains:
• 120 µL Platinum Taq DNA Polymerase
• 3 x 1.25 mL 10X Green PCR Buffer, -Mg
• 1.25 mL 50 mM magnesium chloride
• 1.25 mL KB Extender
Store at -20°C in a non-frost-free freezer.

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