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Gibco™

MEM α, no nucleosides, powder

Catalog number:  12000022

Related applications:

Cell Culture

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Description

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco® MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM α is modified as follows:

With Without
• Phenol Red • Ribonucleosides
• L-glutamine • Deoxyribonucleosides
• Sodium Bicarbonate

The complete formulation is available.

Gibco® MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle’s salts.

cGMP Manufacturing and Quality System
For supply chain continuity, we manufacture Gibco® MEM α at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

Glucose: Low Glucose
HEPES Buffer: No HEPES
Product Line: Gibco®
Quantity: 10 x 1L
Serum Supplementation: Standard Serum Supplementation
Shipping Condition: Room Temperature
Sodium Pyruvate Additive: Sodium Pyruvate
Form: Powder
Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red

Contents & storage

Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture