Invitrogen™
ElectroMAX™ DH10B T1 Phage-Resistant Competent Cells
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Description
ElectroMAX DH10B T1 Phage-Resistant Competent Cells offer transformation efficiencies that are among the highest achieved by any product we offer, and can exceed 1 x 1010 cfu/µg plasmid DNA in efficiency. They are ideal for applications requiring high-efficiency transformation, including cDNA and gDNA library construction. ElectroMAX DH10B cells:• Maximize yield of transformants from limited cloning products
• Permit efficient cloning of methylated DNA
• Are resistant to T1 and T5 bacteriophages
• Support blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
• Deliver high-yield plasmid preparations for downstream applications
Phage-resistant representative libraries from a single transformation
The tonA mutation in DH10B cells confers resistance to bacteriophages T1 and T5, safeguarding valuable libraries against contamination. Additionally, mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr) make ElectroMAX DH10B T1 Phage-Resistant Cells ideal for construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA, and allow for efficient plasmid rescue from eukaryotic genomes. ElectroMAX DH10B T1 Phage-Resistant Cells are suitable for construction of gene banks, for the generation of cDNA libraries using plasmid-derived vectors, and for situations when DNA is limiting. This strain also has the Φ80lacZΔM15 genotype, providing for the option of blue/white screening on plates containing either X-Gal or Bluo-Gal. Finally, the endA1 mutation makes DH10B an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification.
Genotype: F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ-rpsL nupG tonA
Find the strain and format that you need
DH10B cells are available in both electrocompetent and chemically competent formats. Our ElectroMAX DH10B cells are also suitable for cDNA or gDNA library construction but are not resistant to T1 and T5 phage infection. Our MegaX DH10B T1R Electrocomp Cells have the highest transformation efficiency (>3 x 1010 cfu/µg plasmid DNA) and also offer the benefit of T1 and T5 phage resistance.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Competent Cell Type: | Electrocompetent |
|---|---|
| Species: | E. coli |
| Format: | Tube(s) |
| Transformation Efficiency: | > 1x10^10 |
| Bacterial or Yeast Strain: | DH10B |
| Product Line: | DH10B, ElectroMAX™ |
| Blue-White Screening: | Yes |
| Cloning Methylated DNA: | Yes |
| Cloning Unstable DNA: | Not Suitable for cloning unstable DNA |
| F' Episome (to make ssDNA): | Lacks F' Episome |
| High Throughput Compatibility: | Not High Throughput-Compatible (Manual) |
| Improves Plasmid Quality: | Yes |
| Plasmid Type: | May be used for plasmids > 20 kb |
| Preparing Unmethylated DNA: | Not Suitable for preparing unmethylated DNA |
| Product Size: | 5 x 100 µL |
| Propagating ccdB Vectors: | Not for ccdB vector propagation |
| Reduces Recombination: | Yes |
| T1 Phage - Resistant (tonA): | Yes |
| Shipping Condition: | Dry Ice |
Contents & storage
Contains:
• ElectroMAX DH10B T1 Phage-Resistant Cells: 5 vials, 100 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 2 bottles, 6 ml each
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
• ElectroMAX DH10B T1 Phage-Resistant Cells: 5 vials, 100 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 2 bottles, 6 ml each
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
