ElectroMAX™ DH10B™ T1 Phage-Resistant Competent Cells

Description

ElectroMAX™ DH10B™ T1 Phage-Resistant Competent Cells offer transformation efficiencies that are among the highest achieved by any product we offer, and can exceed 1 x 10¹⁰ cfu/µg plasmid DNA in efficiency. They are ideal for applications requiring high-efficiency transformation, including cDNA and gDNA library construction. ElectroMAX™ DH10B™ cells:

• Maximize yield of transformants from limited cloning products
• Permit efficient cloning of methylated DNA
• Are resistant to T1 and T5 bacteriophages
• Support blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
• Deliver high-yield plasmid preparations for downstream applications

Phage-resistant representative libraries from a single transformation
The tonA mutation in DH10B™ cells confers resistance to bacteriophages T1 and T5, safeguarding valuable libraries against contamination. Additionally, mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr) make ElectroMAX™ DH10B™ T1 Phage-Resistant Cells ideal for construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA, and allow for efficient plasmid rescue from eukaryotic genomes. ElectroMAX™ DH10B™ T1 Phage-Resistant Cells are suitable for construction of gene banks, for the generation of cDNA libraries using plasmid-derived vectors, and for situations when DNA is limiting. This strain also has the Φ80lacZΔM15 genotype, providing for the option of blue/white screening on plates containing either X-Gal or Bluo-Gal. Finally, the endA1 mutation makes DH10B™ an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification.

Genotype: F^-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ^-rpsL nupG tonA

Find the strain and format that you need
DH10B™ cells are available in both electrocompetent and chemically competent formats. Our ElectroMAX DH10B™ cells are also suitable for cDNA or gDNA library construction but are not resistant to T1 and T5 phage infection. Our MegaX DH10B™ T1^R Electrocomp™ Cells have the highest transformation efficiency (>3 x 10¹⁰ cfu/µg plasmid DNA) and also offer the benefit of T1 and T5 phage resistance.

Spécifications

Competent Cell Type:	Electrocompetent
Species:	E. coli
Format:	Tube(s)
Transformation Efficiency:	> 1x10^10
Bacterial or Yeast Strain:	DH10B™
Product Line:	DH10B™, ElectroMAX™
Blue-White Screening:	Yes
Cloning Methylated DNA:	Yes
Cloning Unstable DNA:	Not Suitable for cloning unstable DNA
F' Episome (to make ssDNA):	Lacks F' Episome
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Improves Plasmid Quality:	Yes
Plasmid Type:	May be used for plasmids > 20 kb
Preparing Unmethylated DNA:	Not Suitable for preparing unmethylated DNA
Product Size:	25 reactions
Propagating ccdB Vectors:	Not for ccdB vector propagation
Reduces Recombination:	Yes
T1 Phage - Resistant (tonA):	Yes
Shipping Condition:	Dry Ice

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