ElectroMAX DH10B T1 Phage-Resistant Competent Cells offer transformation efficiencies that are among the highest achieved by any product we offer, and can exceed 1 x 10
10 cfu/µg plasmid DNA in efficiency. They are ideal for applications requiring high-efficiency transformation, including cDNA and gDNA library construction. ElectroMAX DH10B cells:
• Maximize yield of transformants from limited cloning products
• Permit efficient cloning of methylated DNA
• Are resistant to T1 and T5 bacteriophages
• Support blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
• Deliver high-yield plasmid preparations for downstream applications
Phage-resistant representative libraries from a single transformationThe
tonA mutation in DH10B cells confers resistance to bacteriophages T1 and T5, safeguarding valuable libraries against contamination. Additionally, mutations in the methylation-dependent restriction system (
mcrA,
mcrBC, and
mrr) make ElectroMAX DH10B T1 Phage-Resistant Cells ideal for construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA, and allow for efficient plasmid rescue from eukaryotic genomes. ElectroMAX DH10B T1 Phage-Resistant Cells are suitable for construction of gene banks, for the generation of cDNA libraries using plasmid-derived vectors, and for situations when DNA is limiting. This strain also has the Φ80
lacZΔM15 genotype, providing for the option of blue/white screening on plates containing either X-Gal or Bluo-Gal. Finally, the
endA1 mutation makes DH10B an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification.
Genotype: F
-mcrA Δ(
mrr-
hsdRMS-
mcrBC) Φ80
lacZΔM15 Δ
lacX74
recA1
endA1
araD139Δ(
ara,
leu)7697
galU
galK λ
-rpsL
nupG
tonA
Find the strain and format that you needDH10B cells are available in both electrocompetent and chemically competent formats. Our
ElectroMAX DH10B cells are also suitable for cDNA or gDNA library construction but are not resistant to T1 and T5 phage infection. Our
MegaX DH10B T1R Electrocomp Cells have the highest transformation efficiency (>3 x 10
10 cfu/µg plasmid DNA) and also offer the benefit of T1 and T5 phage resistance.
For Research Use Only. Not for use in diagnostic procedures.