This product is manufactured in our Grand Island, US facility and is intended for sale in North America, Latin America, and the Asia/Pacific region—higher freight charges will apply to orders shipped elsewhere. An equivalent product, manufactured in Paisley, Scotland, is recommended for our customers in Europe, Africa, and the Middle East. Click to view.

DMEM, powder, high glucose

Catalog number: 12100061

Gibco™  Related applications: Mammalian Cell Culture

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Description

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
WithWithout
• High Glucose• Sodium Pyruvate
• L-glutamine• HEPES
• Phenol Red• Sodium Bicarbonate

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco® cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable DMEM product made in our Scotland facility (52100-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

“This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in specific region, it should be used for laboratory use only.”

Specifications

Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red
Form: Powder
Glucose: High Glucose
Serum Supplementation: Standard Serum Supplementation
HEPES Buffer: No HEPES
Sodium Pyruvate Additive: No Sodium Pyruvate
Product Size: 10 L
Glucose Concentration: 4500 mg/L
Sodium Bicarbonate Buffer: No Sodium Bicarbonate
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture

Documents

Manuals & protocols

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