Invitrogen™

PureLink™ Pro 96 total RNA Purification Kit

Catalog number:  12173011A

Related applications:

RNA Extraction

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Description

The PureLink® Pro 96 total RNA Purification Kit provides high-throughput isolation of high-quality total RNA from the widest range of sample types and sizes. Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples (Figures 1 and 2).
The one-hour procedure requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water; ideal for use in a variety of applications, including RT-PCR, qPCR, northern blotting, and nuclease protection assays.

Improved plate design
The PureLink® Pro 96 total RNA Purification Kit provides optimized semi-skirted 96-well plates(Figure 3) for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at 2250 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Final Product Type: Total RNA
For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting
For Use With (Equipment): Biomek® FX, Robotic Liquid Handling Systems, Vacuum Manifold, Eppendorf epMotion, Centrifuge, TECAN Freedom Evo, TECAN Genesis
Purification Target: Total RNA
Sample Type: Bacteria, Blood Samples, Cell Cultures, Insect Cells, Mammalian Cells, Plant, Tissue (Mammalian), Yeast, Blood
Format: 96-well plate
Isolation Technology: Silica Plate
No. of Reactions: 96 Preps
Quantity: 1 kit
Yield: 33 µg
Elution Volume Max: 150 µl
Elution Volume Min: 50 µl (centrifuge), 100 µl (vacuum)
High-throughput Compatibility: High-throughput Compatible
Purity or Quality Grade: Molecular Biology Grade
Purification Time: 20 min
Scale: Mini
Shipping Condition: Room Temperature
System Type: PureLink™
Product Line: PureLink™

Contents & storage

The PureLink® Pro 96 total RNA Purification Kit is supplied includes PureLink 96 Well Total RNA Filter Plates, elution plates, lysis buffer, Wash Buffers I and II, and RNase-free water. Components are also available separately. Store at room temperature. Guaranteed stable for 2 years when properly stored.