PureLink® RNA Micro Scale Kit

Catalog number: 12183016

Ambion™  Related applications: RNA Extraction | SOLiD® Next-Generation Sequencing

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The PureLink® RNA Micro Scale Kit provides rapid purification and concentration of total RNA from a variety of samples (e.g., LCM tissues, fine needle aspirates, and FACS-sorted cells). RNA purification using the PureLink® RNA Micro Scale Kit is:

Effective—expect excellent RNA recovery (up to 100 µg total RNA) from small samples
Convenient and safe—the extraction procedure uses no phenol/chloroform, CsCl gradients, or precipitation with either LiCl or ethanol
Fast—obtain high-quality total RNA, typically in just 15 minutes
Reliable—obtain ready-to-use RNA suitable for any downstream application

A simple and reliable technology for everyday RNA purification
PureLink® technology combines guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can be completed in typically less than 15 minutes with >85% recovery from the first elution. The kit can be used for clean-up of purified RNA from enzymatic reactions, desalting of RNA samples after phenol extraction or ethanol precipitation, and concentration of RNA. Purify RNA from up to 10 mg of tissue or 105 cells. Clean up and concentrate RNA amounts ranging from pg to 100 µg in volumes as small as 10 µL. Purified RNA is ready for use in downstream applications such as RT-PCR, qRT-PCR, northern blots, cDNA library construction, and nuclease protection assays.

A simple and rapid procedure
Homogenize your sample in lysis buffer, add 70% ethanol, and mix. Deposit the resulting solution into a PureLink® Micro kit column with collection tube, and centrifuge for one minute. Nucleic acids bind to the PureLink® silica membrane in the column, and after subsequent washes and on-column digestion with DNase, purified RNA can be eluted with RNase-free water.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Liquid Samples (e.g. Serum), Plant Samples, Bacteria, Yeast, Tissue, Cells
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Nucleic Acid Labeling, Nuclease Protection Assays, Real-Time Quantitative PCR (qPCR), Northern Blotting
Scale: Micro
Format: Spin Column
System: PureLink™
Product Size: 50 preps
Final Product: Total RNA
Yield (up to): 100 µg
Purification Time: 15 min
Elution Volume - max: 10 µl
Elution Volume - min: 5 µl
Isolation Technology: Silica Spin Column
Sample Type (Specific): Bacteria, Laser Capture Microdissection (LCM), Yeast, Tissue (Mammalian), Plant, Fine Needle Aspirates (FNA), Mammalian Cells
Purity or Quality Grade: Molecular Biology Grade
Starting Material (Amount): Up to 5 x 10^5 cells, Less than 10 mgs tissue
Shipping Condition: Room Temperature

Contents & storage

Box 1 (PureLink™ RNA Micro Kit):
• Lysis Buffer (125 ml)
• Wash Buffer I (50 ml)
• Wash Buffer II (15 ml)
• RNase-Free Water (15.5 ml)
• PureLink™ Micro Kit Columns with collection tubes (50 each)
• Collection Tubes (50 each)
• Recovery Tubes (50 each)

Box 2 (PureLink™ DNase/Carrier RNA)
• Lyophilized PureLink™ DNase (1500 Units)
• PureLink™ On-Column DNase 2X Buffer (1 ml)
• RNase-Free Water (1 ml)
• Lyophilized PureLink™ Carrier RNA (300 µg)

Store Box 2 at 4°C. Store remaining components at room temperature.

Documents

Manuals & protocols

Recommended products