PureLink® RNA Mini Kit

Catalog number: 12183025

Ambion™  Related applications: RNA Extraction | SOLiD® Next-Generation Sequencing

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The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. The PureLink® RNA Mini Kit provides:

• Rapid, non-hazardous RNA purification capabilities
• Ready-to-use RNA for many downstream applications

Rapid and convenient column purification
The PureLink® RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1,000 µg of purified RNA from a single extraction (see table). High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with no genomic DNA contamination.

Simplified, nontoxic RNA purification
The PureLink® RNA Mini Kit combines nontoxic guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can typically be completed in less than 20 minutes without the need for hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl, or alcohol precipitation.

The PureLink RNA Mini Kit is recommended to be used together with the Homogenizer (Cat. No. 12183026) designed to homogenize cell or tissue lysates via centrifugation, prior to nucleic acid purification. The Homogenizer is especially effective for clarifying particulates from plant tissues.

For plant tissues rich in polyphenolics or starch (e.g., pine needles, potato tubers), we recommend using PureLink™ Plant RNA Reagent (Cat. No. 12322012) together with the PureLink RNA Mini Kit.

For Research Use Only. Not for use in diagnostic procedures.


Sample Type (General): Liquid Samples (e.g. Serum), Plant Samples, Tissue, Bacteria, Yeast, Cells, Blood
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Reverse Transcriptase PCR (RT-PCR), Real-Time Quantitative PCR (qPCR), Next-Generation Sequencing, Microarray Analysis, Nuclease Protection Assays, Northern Blotting
Scale: Mini
Format: Spin Column
System: PureLink™
Product Size: 250 preps
Final Product: Total RNA
Yield (up to): 350 µg
Purification Time: 20 min
Elution Volume - max: 100 µl
Elution Volume - min: 30 µl
Isolation Technology: Silica Spin Column
Sample Type (Specific): Tissue Homogenates, Bacterial Culture, Plant, Any Tissue Type, Yeast, Cell Cultures
Starting Material (Amount): Up to 200 mg of tissue, 5x10^6 to 1x10^8 cells
Shipping Condition: Room Temperature

Contents & storage

• Lysis Buffer (500 ml)
• Wash Buffer I (250 ml)
• Wash Buffer II (75 ml)
• RNase-Free Water (75 ml)
• Spin Cartridges with collection tubes (5 × 50 each)
• Collection Tubes (5 × 50 each)
• Recovery Tubes (5 × 50 each)

Store at room temperature.


Manuals & protocols

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