TRIzol® Plus RNA Purification Kit

Catalog number: 12183555

Ambion™  Related applications: Microarray Analysis | RNA Extraction | miRNA & Non-Coding RNA Analysis

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Description

The TRIzol® Plus RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a wide variety of samples, including animal and plant cells, tissue, bacteria, and yeast. The kit combines the strong lysis capability of TRIzol® Reagent, followed by a convenient and time-saving silica-cartridge purification protocol from the PureLink® RNA Mini Kit, to provide ultrapure total RNA typically within an hour, even from difficult samples such as fibrous or fatty tissues.

Features of this kit include:

• Combined TRIzol®-based lysis and PureLink™ spin column-based RNA isolation technologies
• Superior lysis capability and convenient protocol

Two products in one
The TRIzol® Plus RNA Purification System couples the lysis capabilities of TRIzol® Reagent with the 1000 µg column-;binding capacity of the PureLink™ RNA Mini Kit, resulting in high RNA integrity and yield. The TRIzol® Plus RNA Purification System includes protocols for a variety of sample types, including fibrous or fatty tissues and plant material. The combination of TRIzol® Reagent followed by silica-cartridge purification of RNA is the recommended RNA purification method in the gene expression industry. The TRIzol® Plus RNA Purification Kit provides the reagents and an optimized protocol to purify total RNA for gene expression studies using an industry recommended method.

Simple lysis to column purification protocol
TRIzol® Reagent sample lysis maintains the integrity of the RNA, while disrupting cells and dissolving cell components. TRIzol® Reagent also provides an immediate and highly effective inhibition of RNase activity during sample homogenization. Following lysis, RNA in the lysed sample is bound to the clear silica-based membrane in the PureLink™ RNA Mini Kit Spin Cartridge, washed to remove contaminants, and then eluted in RNase-Free water (Tris Buffer, pH 7.5 may also be used).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Bacteria, Plant Samples, Tissue, Yeast, Cells
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Nucleic Acid Labeling, Microarray Analysis, Real-Time Quantitative PCR (qPCR), Northern Blotting, cDNA Library Construction
Scale: Mini
Format: Spin Column
System: TRIzol
Quantity: 50
Product Size: 50 preps
Final Product: Total RNA
Yield (up to): 1000 µg
Purification Time: 20 min
Number of Reactions: 50 Preps
Isolation Technology: Organic Extraction, Silica Spin Column
Starting Material (Amount): Up to 200 mg tissue, Up to 5 x 10^8 cells

Contents & storage

Kit Components:
• TRIzol® Reagent (100 ml)
• Lysis Buffer (125 ml)
• Wash Buffer I (50 ml)
• Wash Buffer II (15 ml)
• RNase-Free Water (15.5 ml)
• Spin Cartridges with collection tubes (50 each)
• Collection Tubes (50 each)
• Recovery Tubes (50 each)

Store all components at room temperature. Sufficient reagents are provided to perform 50 RNA isolations.

Documents

Manuals & protocols

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