Informational:Inquire about OEM or Commercial Supply version of this product here.
Invitrogen™

TRIzol™ Plus RNA Purification Kit

Catalog number: 12183555
Invitrogen™

TRIzol™ Plus RNA Purification Kit

Catalog number: 12183555
Catalog Number
12183555
also known as 12183-555
Unit Size
50 preps
Price (USD)
Price: 424.00
Online price: 395.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12183555
also known as 12183-555
50 preps
Price: 424.00
Online price: 395.65
Your price:
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations
The TRIzol™ Plus RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a wide variety of samples, including animal and plant cells, tissue, bacteria, and yeast. The kit combines the strong lysis capability of TRIzol™ Reagent, followed by a convenient and time-saving silica-cartridge purification protocol from the PureLink™ RNA Mini Kit, to provide ultrapure total RNA typically within an hour, even from difficult samples such as fibrous or fatty tissues.

Features of this kit include:

• Combined TRIzol™-based lysis and PureLink™ spin column-based RNA isolation technologies
• Superior lysis capability and convenient protocol

Two products in one
The TRIzol™ Plus RNA Purification System couples the lysis capabilities of TRIzol™ Reagent with the 1000 μg column-;binding capacity of the PureLink™ RNA Mini Kit, resulting in high RNA integrity and yield. The TRIzol™ Plus RNA Purification System includes protocols for a variety of sample types, including fibrous or fatty tissues and plant material. The combination of TRIzol™ Reagent followed by silica-cartridge purification of RNA is the recommended RNA purification method in the gene expression industry. The TRIzol™ Plus RNA Purification Kit provides the reagents and an optimized protocol to purify total RNA for gene expression studies using an industry recommended method.

Simple lysis to column purification protocol
TRIzol™ Reagent sample lysis maintains the integrity of the RNA, while disrupting cells and dissolving cell components. TRIzol™ Reagent also provides an immediate and highly effective inhibition of RNase activity during sample homogenization. Following lysis, RNA in the lysed sample is bound to the clear silica-based membrane in the PureLink™ RNA Mini Kit Spin Cartridge, washed to remove contaminants, and then eluted in RNase-Free water (Tris Buffer, pH 7.5 may also be used).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Isolation Technology
Silica Spin Column, Organic Extraction
Purification Time
1 hr.
Elution Volume
30 to 300 μL
Sample Type
Bacteria, Cells, Plant Samples, Tissue, Yeast
Final Product Type
Total RNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
For Use With (Application)
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
Quantity
50 Preps
Scale
Mini
Shipping Condition
Room Temperature
Starting Material Amount
Bacteria: ≤107
Cells: ≤107
Plant: ≤107, ≤100 mg
Tissue: ≤100 mg
Yeast: ≤107
Yield
Up to 350 μg

Contents & Storage

• 100 mL TRIzol Reagent; room temperature
• 1 PureLink RNA Mini Kit: 125 mL Lysis Buffer; room temperature
• 50 mL Wash Buffer I; room temperature
• 15 mL Wash Buffer II; room temperature
• 15.5 mL RNase-free Water; room temperature
• 50 PureLink Spin Cartridges with Collection Tubes; room temperature
• 50 Collection Tubes; room temperature
• 50 Recovery Tubes; room temperature

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text