CD44 Recombinant Human Protein, His Tag

Description

Manufactured by Sino Biological (China); Distributed by Life Technologies.

Cluster of Differentiation 44 (CD44) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant human protein is expressed from a DNA sequence encoding the extracellular domain (Met 1-Pro 220) of human CD44 (P16070-1) fused to a polyhistidine tag at the C-terminus.

N-terminal Sequence Analysis: Gln 21.

Activity: this recombinant protein has not been tested.

Formulation: lyophilized in PBS, pH 7.4, 5% mannitol, 5% trehalose, and 0.02% Tween®-80.

Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: expires one year from date of receipt when stored as instructed.

Background
CD44, short for Cluster of Differentiation 44, is an adhesion molecule of the hyaluronate receptor family. The adhesive function of CD44, like other molecules involved in inducible adhesion, is tightly regulated. Posttranslational modifications, isoform expression, aggregation state, and protein associations all can affect the ligand binding properties of CD44, and these can vary depending on the cell type and the activation state of the cell. The principal ligand of CD44 is hyaluronan, and CD44 is involved in the metabolism of this compound. Furthermore, an increasing quantity of evidence suggests that CD44 has various functions related to inflammatory disease. CD44 deficiency induces severe liver injury. CD44-hyaluronate mediates in lymphocyte T and monocyte adhesion to the endothelium, stimulates proinflammatory cytokine release from macrophages and participates in dedifferentiation phenotype of smooth muscle cells from contractile state to synthetic one. CD44 acts in atherosclerotic plaque destabilization and neointimal proliferation after percutaneous vascular interventions. CD44 participates in many stages of atherosclerosis. CD44 also is a cell membrane protein often overexpressed on tumor cells and, being both a cell-cell and cellextracellular matrix adhesion protein, is well positioned to contribute to this process.

You may also be interested in the following CD44 products:
Primary Antibodies
Gene Expression Assays

Figures

Specifications

Protein Family:	CD Proteins
Research Category:	Extracellular Matrix & Cell Adhesion, Immunology
Endotoxin Level:	< 1 EU/µg
Expression:	Mammalian
Form:	Lyophilized
Gene Aliases:	AL133330.1, CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MGC10468
Gene ID:	960
Gene Symbol:	CD44
Molecular Weight:	23.4 kDa
Product Line:	Sino Biological®
Product Size:	50 µg
Protein Form:	Cell Surface Protein, Recombinant, Truncated
Protein Name:	CD44 molecule (Indian blood group)
Protein Subtype:	Glycoproteins
Protein Tag or Fusion:	His Tag
Purity:	≥ 90% by SDS-PAGE
Species:	Human
Validated Application:	Assay Standard
Shipping Condition:	Room Temperature

Contents & storage