Invitrogen™

Gateway™ pET-DEST42 Vector

Catalog number:  12276010

Related applications:

Gateway Cloning

| Protein Expression

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Description

The Champion™ pET-DEST42 Gateway® destination vector is designed for rapid cloning with a Gateway® entry clone using lambda phage site-specific recombination and subsequent high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by the production of T7 RNA polymerase in BL21(DE3) E. coli. The Champion™ pET-DEST42 destination vector offers:

• Bacteriophage T7lac promoter for high-level expression
O operator sequences for lac repressor binding to ensure tighter regulation of transcription
• pBR322 ori for minimal basal expression
• C-terminal V5 and 6xHis tags for efficient detection and purification
R sites for efficient recombination with any attL-flanked Gateway® entry vector
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Antibiotic Resistance (Bacterial): Ampicillin (AmpR)
Cloning Method: Gateway®
Constitutive or Inducible System: Inducible
Inducing Agent: IPTG
Product Line: Gateway®
Product Size: 6 µg
Promoter: T7 and lacO
Protein Tag or Fusion: His Tag (6x),V5 Epitope Tag
Selection Agent (Eukaryotic): None
Vector Type: pDEST,pET

Contents & storage

The Champion™ pET-DEST42 Gateway® destination vector includes 6 µg of vector. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.