The Champion™ pET-DEST42 Gateway™ destination vector is designed for rapid cloning with a Gateway™ entry clone using lambda phage site-specific recombination and subsequent high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by the production of T7 RNA polymerase in BL21(DE3) E. coli. The Champion™ pET-DEST42 destination vector offers:
• Bacteriophage T7lac promoter for high-level expression O operator sequences for lac repressor binding to ensure tighter regulation of transcription • pBR322 ori for minimal basal expression • C-terminal V5 and 6xHis tags for efficient detection and purification R sites for efficient recombination with any attL-flanked Gateway™ entry vector
For Research Use Only. Not for use in diagnostic procedures.
Constitutive or Inducible System
Gateway System Destination Expression Vector
Selection Agent (Eukaryotic)
Antibiotic Resistance Bacterial
His Tag (6x), V5 Epitope Tag
Contents & Storage
The Champion™ pET-DEST42 Gateway™ destination vector includes 6 µg of vector. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.