PureLink™ Viral RNA/DNA Mini-Kit

Yes, the Cat. No. for 100 wash tubes is 12282-100.

The Proteinase K solution is stable for 1 year when stored at room temperature. For long-term storage (>1 year) or if room temperature is >25 degrees C, store the Proteinase K solution at 4 degrees C.

Plasma is the liquid portion of the blood that is separated from the blood cells by centrifugation. One of the characteristics of plasma is that it clots easily, which is important for hemophiliacs needing a transfusion but is a nuisance in most other applications. By agitating the plasma, one can precipitate the clotting factors as a large clot, and the leftover fluid is called serum. In other words, serum plus clotting factors is plasma, and clotted plasma yields serum.

The tRNA is stable for over one year. There was no significant difference in qRT-PCR results with tRNA stored for 10 days at ‘20 degrees C or 42 degrees C. The lyophilized tRNA is stable at ‘20 degrees C, room temperature, 37 degrees C, and 45 degrees C for up to 1 month.

The carrier RNA is tRNA and it may not be necessary. We have tested without the tRNA and obtained excellent results. The tRNA provides a substrate for RNases in the sample to protect the viral RNA until the virus is lysed. You may want to scale down the amount of carrier RNA or leave it out. However, you should validate it in your application to make sure you get the same results as when you include it.