PureLink™ Viral RNA/DNA Mini Kit

Catalog number:  12280050

Invitrogen™  Related applications: DNA Extraction | RNA Extraction | Viral RNA⁄DNA Purification

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The PureLink® Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants. The PureLink® Viral Mini Kit is specifically designed to isolate high-quality viral nucleic acids from a variety of RNA and DNA viruses within 45 minutes using low elution volumes that allow sensitive downstream analysis. The purified viral RNA/DNA is devoid of proteins and nucleases, and is suitable for use in downstream applications that allow viral detection and genotyping. The PureLink® Viral RNA/DNA Mini Kit provides the following advantages:

• Rapid and efficient purification of high-quality viral nucleic acid using spin column–based centrifugation with no sample cross-contamination
• Specifically designed to purify viral RNA and DNA from ≤500 µL cell-free samples within 45 min
• Ability to elute viral nucleic acids in low elution volumes of 10–50 µL to allow sensitive downstream analysis
For Research Use Only. Not for use in diagnostic procedures.



LoyaltyEligible: SKUEligibleForLoyalty
Sample Type (General): Liquid Samples (e.g. Serum), Viral Samples
Sample Type (Specific): Cell-Free Fluids, Cerebrospinal Fluid, Plasma, Serum
Downstream Application: Northern Blotting, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Isolation Technology: Silica Spin Column
Product Line: PureLink™
Product Size: 50 preps
Shipping Condition: Room Temperature

Contents & storage

The PureLink® Viral RNA/DNA Mini Kit contains enough reagents for 50 reactions.

Kit Contents:
• 32 ml Viral Lysis buffer (L22)
• 15 ml 5X Wash Buffer (W5)
• 2 × 1.6 ml Proteinase K (20 mg/ml in storage buffer)
• 310 µg lyophilized Carrier RNA
• 15.5 ml Sterile, RNAase-free water
• 50 Viral Spin Columns with Collection Tubes
• 2 × 50 Wash Tubes (2.0 ml)
• 50 Recovery Tubes (1.5 ml)

Store all components except for Carrier RNA at room temperature. Carrier RNA should be stored at −20°C.


Manuals & protocols

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