PureLink® Viral RNA/DNA Mini Kit

Catalog number: 12280050

Invitrogen™  Related applications: DNA Extraction | RNA Extraction | Viral RNA⁄DNA Purification

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Description

The PureLink® Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants. The PureLink® Viral Mini Kit is specifically designed to isolate high-quality viral nucleic acids from a variety of RNA and DNA viruses within 45 minutes using low elution volumes that allow sensitive downstream analysis. The purified viral RNA/DNA is devoid of proteins and nucleases, and is suitable for use in downstream applications that allow viral detection and genotyping. The PureLink® Viral RNA/DNA Mini Kit provides the following advantages:

• Rapid and efficient purification of high-quality viral nucleic acid using spin column–based centrifugation with no sample cross-contamination
• Specifically designed to purify viral RNA and DNA from ≤500 µL cell-free samples within 45 min
• Ability to elute viral nucleic acids in low elution volumes of 10–50 µL to allow sensitive downstream analysis
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Liquid Samples (e.g. Serum), Viral Samples
Sample Type (Specific): Plasma, Serum, Cell-Free Fluids, Cerebrospinal Fluid
Downstream Application: Reverse Transcriptase PCR (RT-PCR), Northern Blotting, Real-Time Quantitative PCR (qPCR)
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Isolation Technology: Silica Spin Column
Product Size: 50 preps
Shipping Condition: Room Temperature

Contents & storage

The PureLink® Viral RNA/DNA Mini Kit contains enough reagents for 50 reactions.

Kit Contents:
• 32 ml Viral Lysis buffer (L22)
• 15 ml 5X Wash Buffer (W5)
• 2 × 1.6 ml Proteinase K (20 mg/ml in storage buffer)
• 310 µg lyophilized Carrier RNA
• 15.5 ml Sterile, RNAase-free water
• 50 Viral Spin Columns with Collection Tubes
• 2 × 50 Wash Tubes (2.0 ml)
• 50 Recovery Tubes (1.5 ml)

Store all components except for Carrier RNA at room temperature. Carrier RNA should be stored at −20°C.

Documents

Manuals & protocols

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