The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The Gateway™ pcDNA™-DEST47 vector offers the following key features:

•C-terminal Cycle 3 GFP tag for rapid detection of recombinant protein
•Cytomegalovirus (CMV) promoter for high-level expression
•attR sites for Gateway™ cloning, enabling recombination with attL-flanked fragments
•Neomycin resistance gene for stable selection
•Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

Gateway™ Cloning
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ ORF Clone. The following table lists a variety of available destination vectors.

Additional materials required, available separately: Gateway™ entry clone, appropriate Gateway™ LR Clonase™ enzyme mix, and reaction buffer.