pBAD-DEST49 Gateway™ Destination Vector

Citations & References (2)

Invitrogen™

pBAD-DEST49 Gateway™ Destination Vector

The pBAD-DEST49 Gateway™ destination vector is designed for rapid cloning with a Gateway™ entry clone using lambda phage site-specific recombinationRead more

Catalog Number	Quantity
12283016 also known as 12283-016	6 μg

Catalog number 12283016

also known as 12283-016

Price (MXN)

Quantity:

The pBAD-DEST49 Gateway™ destination vector is designed for rapid cloning with a Gateway™ entry clone using lambda phage site-specific recombination and subsequent high-level, tightly regulated expression in E. coli. The pBAD-DEST49 vector features:

• The araBAD promoter for tightly regulated expression in E. coli
• HP-thioredoxin fusion for improved protein yield and solubility
• Enterokinase cleavage site for efficient cleavage of the N-terminal fusion with EKMax™
• C-terminal V5 and 6xHis tags for efficient detection and purification of fusion proteins
• R sites for efficient recombination with any attL-flanked Gateway™ entry vector

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Antibiotic Resistance BacterialAmpicillin (AmpR)

CleavageEK (Enterokinase) Recognition Site

Constitutive or Inducible SystemInducible

Inducing AgentArabinose

Product TypeGateway System Destination Expression Vector

Quantity6 μg

Selection Agent (Eukaryotic)None

VectorpBAD, pDEST

Cloning MethodGateway

Product LineGateway

PromoteraraBAD

Protein TagHis Tag (6x), V5 Epitope Tag, Thioredoxin

Unit SizeEach

Contents & Storage

The pBAD-DEST49 Gateway™ destination vector includes 6 μg of vector. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.

Frequently asked questions (FAQs)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all pBAD-DEST49 Gateway™ Destination Vector FAQs

Citations & References (2)

Citations & References

Abstract

Mmh/Ogg1 gene inactivation results in accumulation of 8-hydroxyguanine in mice

Authors:Minowa O, Arai T, Hirano M, Monden Y, Nakai S, Fukuda M, Itoh M, Takano H, Hippou Y, Aburatani H, Masumura K, Nohmi T, Nishimura S, Noda T

Journal:Proc Natl Acad Sci U S A

PubMed ID:10725358

The major mutagenic base lesion in DNA caused by exposure to reactive oxygen species is 8-hydroxyguanine or 7, 8-dihydro-8-oxoguanine (8-OH-G). Products of the human MMH/OGG1 gene are known to catalyze in vitro the reactions repairing this DNA lesion. To analyze the function of Mmh in vivo, we generated a mouse ... More

High throughput immuno-screening of cDNA expression libraries produced by in vitro recombination; exploring the Plasmodium falciparum proteome.

Authors:Kordai Sowa MP, Sharling L, Humphreys G, Cavanagh DR, Gregory WF, Fenn K, Creasey AM, Arnot DE,

Journal:Mol Biochem Parasitol

PubMed ID:14698438

Improved Plasmodium falciparum cDNA expression libraries were constructed by combining mRNA oligo-capping with in vitro recombination and directional cloning of cDNA inserts into a plasmid vector that expresses sequences as thioredoxin fusion proteins. A novel procedure has also been developed for the rapid identification of seropositive clones on high-density filters, ... More