pBAD-DEST49 Gateway™ Destination Vector

Catalog number:  12283016

 Related applications:

Gateway Cloning

| Protein Expression

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The pBAD-DEST49 Gateway® destination vector is designed for rapid cloning with a Gateway® entry clone using lambda phage site-specific recombination and subsequent high-level, tightly regulated expression in E. coli. The pBAD-DEST49 vector features:

• The araBAD promoter for tightly regulated expression in E. coli
• HP-thioredoxin fusion for improved protein yield and solubility
• Enterokinase cleavage site for efficient cleavage of the N-terminal fusion with EKMax™
• C-terminal V5 and 6xHis tags for efficient detection and purification of fusion proteins
• R sites for efficient recombination with any attL-flanked Gateway® entry vector
For Research Use Only. Not for use in diagnostic procedures.


Vector Type: pBAD, pDEST
Cloning Method: Gateway®
Promoter: araBAD
Protein Tag or Fusion: His Tag (6x), Thioredoxin, V5 Epitope Tag
Antibiotic Resistance (Bacterial): Ampicillin (AmpR)
Cleavage: EK (Enterokinase) Recognition Site
Constitutive or Inducible System: Inducible
Inducing Agent: Arabinose
Product Line: Gateway®
Product Size: 6 µg
Selection Agent (Eukaryotic): None

Contents & storage

The pBAD-DEST49 Gateway® destination vector includes 6 µg of vector. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.


Manuals & protocols